Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics
文献类型：期刊论文
| 作者 | Yin, Nana1; Lin, Bo1; Huo, Feng2; Shu, Yang1; Wang, Jianhua1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2022-08-24 |
| 页码 | 9 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.2c02106 |
| 英文摘要 | Temperature dynamics reflect the physiological state of cells, and accurate measurement of intracellular temperature helps to understand the biological processes. Herein, we report a novel nanothermometer by conjugating a fluorescent probe 3-ethyl-2-[4-(1,2,2-triphenylvinyl)styryl]-benzothiazol-3-ium iodide (TPEBT) with a thermoresponsive polymer poly(N-isopropylacrylamide-co-tetrabutylphosphonium styrenesulfonate) [P(NIPAM-co-TPSS)]. The derived nanoprobe TPEBT-P(NIPAM-co-TPSS) self-assembles into micelles with TPEBT as hydrophobic core and PNIPAM as hydrophilic shell. It exhibits aggregation-induced emission (AIE) at lambda(ex)/lambda(em) = 420/640 nm in aqueous medium with a quantum yield of phi(F) 11.9%. The rise in temperature transforms PNIPAM chains from linear to compact spheres to serve as the core of micelles, and meanwhile converts TPEBT from the state of aggregation to dispersion and redistributes in the micellar shell. Temperature driven phase transition of P(NIPAM-co-TPSS) mediates the reversible aggregation and disaggregation of TPEBT and endows the nanothermometer with temperature-dependent AIE features and favorable sensitivity for temperature sensing in 32-40 ?. TPEBT-P(NIPAM-co-TPSS) is taken up by HeLa cells to distribute mainly in lysosomes. It enables quantitative visualization of in situ thermal dynamics in response to stimuli from carbonyl cyanide 4(trifluoromethoxy)phenylhydrazone, oligomycin, genipin, and lipopolysaccharide. The real-time monitoring of photothermal-induced intracellular temperature variation is further conducted. |
| 资助项目 | Natural Science Foundation of China[21974018] ; Natural Science Foundation of China[22174013] ; Natural Science Foundation of China[22074011] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000848534500001 |
| 资助机构 | Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54539]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Shu, Yang; Wang, Jianhua |
| 作者单位 | 1.Northeastern Univ, Coll Sci, Dept Chem, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Nana,Lin, Bo,Huo, Feng,et al. Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics[J]. ANALYTICAL CHEMISTRY,2022:9. |
| APA | Yin, Nana,Lin, Bo,Huo, Feng,Shu, Yang,&Wang, Jianhua.(2022).Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics.ANALYTICAL CHEMISTRY,9. |
| MLA | Yin, Nana,et al."Nanothermometer with Temperature Induced Reversible Emission for Evaluation of Intracellular Thermal Dynamics".ANALYTICAL CHEMISTRY (2022):9. |
入库方式： OAI收割
来源：过程工程研究所
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