Chitosan hydrochloride salt stabilized emulsion as vaccine adjuvant
文献类型：期刊论文
| 作者 | Wang, Danyang1,2; Zou, Yongjuan1; Wang, Ning2; Wu, Jie1 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2022-11-15 |
| 卷号 | 296页码:10 |
| 关键词 | Adjuvant Chitosan hydrochloride salt Emulsion Cellular immunity Vaccine |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2022.119879 |
| 英文摘要 | Traditional emulsion adjuvants are stabilized by non-ionic surfactants and promote high humoral immunity. However, because of their electroneutrality, they cannot effectively carry negatively charged antigens and enter the cell to elicit cellular immunity. To overcome this problem, we developed an emulsion using chitosan hydrochloride salt (CSCL) as stabilizer. After optimization, CSCL was successfully adsorbed on the surface of the emulsion (CPSE), and stabilized for two months. CPSE could effectively loading the antigen and slow down the rate of the antigen release. We evaluated the effectiveness of CPSE through intramuscular immunization in mice after cytotoxicity assay proved its safety. CPSE not only induced strong antibody-mediated immune responses, but also generated memory T cells in the spleen as a marker of adaptive immunity. Importantly, CPSE showed advantages over the conventional vaccine formulations in promoting IFN-gamma secretion. CPSE may act as a promising candidate to enhance the efficacy of vaccines with acceptable biocompatibility and safety. |
| WOS关键词 | TRIMETHYL CHITOSAN ; NANOPARTICLES ; DELIVERY ; MF59 ; MECHANISM ; RESPONSES ; ANTIBODY ; SQUALENE ; IMMUNITY |
| 资助项目 | National Natural Science Foundation of China (NSFC)[22078335] ; National Natural Science Foundation of China (NSFC)[21776287] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000843937700005 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Natural Science Foundation of China (NSFC) |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54591]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wu, Jie |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol Sch, Minist Educ, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Danyang,Zou, Yongjuan,Wang, Ning,et al. Chitosan hydrochloride salt stabilized emulsion as vaccine adjuvant[J]. CARBOHYDRATE POLYMERS,2022,296:10. |
| APA | Wang, Danyang,Zou, Yongjuan,Wang, Ning,&Wu, Jie.(2022).Chitosan hydrochloride salt stabilized emulsion as vaccine adjuvant.CARBOHYDRATE POLYMERS,296,10. |
| MLA | Wang, Danyang,et al."Chitosan hydrochloride salt stabilized emulsion as vaccine adjuvant".CARBOHYDRATE POLYMERS 296(2022):10. |
入库方式： OAI收割
来源：过程工程研究所
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