Oil-in-ionic liquid nanoemulsion-based adjuvant simultaneously enhances the stability and immune responses of inactivated foot-and-mouth disease virus
文献类型：期刊论文
| 作者 | Lin, Xuan1; Yang, Yanli1; Li, Shuai1; Li, Zhengjun1; Sheng, Yanan1,2; Su, Zhiguo1; Zhang, Songping1 |
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2022-09-25 |
| 卷号 | 625页码:11 |
| ISSN号 | 0378-5173 |
| 关键词 | Ionic liquids Nanoemulsion Adjuvant Stability Inactivated foot-and-mouth disease virus |
| DOI | 10.1016/j.ijpharm.2022.122083 |
| 英文摘要 | Maintaining structural integrity and enhancing stability of inactivated foot-and-mouth disease virus (iFMDV) antigen in adjuvants is crucial to ensure the vaccine potency. Unfortunately, formulation with most reported adjuvants leads to the accelerated dissociation of iFMDV into inactive pentamers. Here, an ionic liquid, i.e., choline and niacin ([Cho][Nic]), which was found to stabilize iFMDV against the acid- and thermo- induced dissociation in buffer solution, was applied to construct a novel oil-in-ionic liquid (o/IL) nanoemulsion adjuvant composed of [Cho][Nic], squalene, and Tween 80. The o/IL nanoemulsion formulated with iFMDV has a monodisperse diameter of 135.8 +/- 40.4 nm. The thermostability and long-term stability of iFMDV were remarkably enhanced in o/IL nanoemulsion compared with that in the o/w emulsion without [Cho][Nic] and in the commercial Montanide ISA 206 adjuvant. The o/IL nanoemulsion exerted its adjuvant effects by improving the humoral immune responses. Immunization of o/IL nanoemulsion adjuvanted iFMDV induced specific IgG titers similar to that adjuvanted by Montanide ISA 206 and about 4-fold higher than the un-adjuvanted iFMDV, also promoted the activation of B lymphocytes and the secretion of interleukin-4 in the mice model. This [Cho] [Nic]-based o/IL nanoemulsion can serve as a promising adjuvant platform for the foot-and-mouth disease vaccine. |
| WOS关键词 | MULTIPLE EMULSIONS ; PHYSICAL STABILITY ; CELL RESPONSES ; DELIVERY ; SIZE ; DISSOCIATION ; MECHANISM ; VACCINES ; PROVIDES |
| 资助项目 | National Natural Science Foundation of China[31970872] ; National Natural Science Foundation of China[21821005] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000855333600006 |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54662]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhang, Songping |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Xuan,Yang, Yanli,Li, Shuai,et al. Oil-in-ionic liquid nanoemulsion-based adjuvant simultaneously enhances the stability and immune responses of inactivated foot-and-mouth disease virus[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2022,625:11. |
| APA | Lin, Xuan.,Yang, Yanli.,Li, Shuai.,Li, Zhengjun.,Sheng, Yanan.,...&Zhang, Songping.(2022).Oil-in-ionic liquid nanoemulsion-based adjuvant simultaneously enhances the stability and immune responses of inactivated foot-and-mouth disease virus.INTERNATIONAL JOURNAL OF PHARMACEUTICS,625,11. |
| MLA | Lin, Xuan,et al."Oil-in-ionic liquid nanoemulsion-based adjuvant simultaneously enhances the stability and immune responses of inactivated foot-and-mouth disease virus".INTERNATIONAL JOURNAL OF PHARMACEUTICS 625(2022):11. |
入库方式： OAI收割
来源：过程工程研究所
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