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Identification and Characterization of Fibronectin-Binding Peptides in Gelatin

文献类型：期刊论文

作者Liu, Yuying1,2; Gao, Jianping1,2; Liu, Lin3; Kang, Jiyao1; Luo, Xi1; Kong, Yingjun1; Zhang, Guifeng1,2
刊名POLYMERS
出版日期2022-09-01
卷号14期号:18页码:16
关键词collagen gelatin fibronectin-binding peptides HPLC-MS
DOI10.3390/polym14183757
英文摘要Collagen and fibronectin (FN) are important components in the extracellular matrix (ECM). Collagen-FN binding belongs to protein-protein interaction and plays a key role in regulating cell behaviors. In this study, FN-binding peptides were isolated from gelatin (degraded collagen) using affinity chromatography, and the amino acid sequences were determined using HPLC-MS. The results indicated that all FN-binding peptides contained GPAG or GPPG. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and dual-polarization interferometry (DPI) were used to analyze the effects of hydroxylation polypeptide on FN binding activity. DPI analysis indicated that peptides with molecular weight (MW) between 2 kDa and 30 kDa showed higher FN-binding activity, indicating MW range played an important role in the interaction between FN and peptides. Finally, two peptides with similar sequences except for hydroxylation of prolines were synthesized. The FN-binding properties of the synthesized peptides were determined by MALDI-TOF MS. For peptide, GAPGADGP*AGAPGTP*GPQGIAGQR, hydroxylation of P8 and P15 is necessary for FN-binding. For peptide, GPPGPMGPPGLAGPPGESGR, the FN-binding process is independent of proline hydroxylation. Thus, FN-binding properties are proline-hydroxylation dependent.
WOS关键词COLLAGEN ; DIFFERENTIATE ; SITES
资助项目National Key Technology R&D Programs of China[2021YFC2400804] ; Open Funding Project of the State Key Laboratory of Biochemical Engineering[2021KF-04] ; Independent Research Project of the State Key Laboratory of Biochemical Engineering[2021ZZ-03]
WOS研究方向Polymer Science
语种英语
出版者MDPI
WOS记录号WOS:000858772700001
资助机构National Key Technology R&D Programs of China ; Open Funding Project of the State Key Laboratory of Biochemical Engineering ; Independent Research Project of the State Key Laboratory of Biochemical Engineering
源URL[http://ir.ipe.ac.cn/handle/122111/54697]  
专题中国科学院过程工程研究所
通讯作者Zhang, Guifeng
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing 100049, Peoples R China
3.Lib Shandong Agr Univ, Tai An 271018, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Yuying,Gao, Jianping,Liu, Lin,et al. Identification and Characterization of Fibronectin-Binding Peptides in Gelatin[J]. POLYMERS,2022,14(18):16.
APA Liu, Yuying.,Gao, Jianping.,Liu, Lin.,Kang, Jiyao.,Luo, Xi.,...&Zhang, Guifeng.(2022).Identification and Characterization of Fibronectin-Binding Peptides in Gelatin.POLYMERS,14(18),16.
MLA Liu, Yuying,et al."Identification and Characterization of Fibronectin-Binding Peptides in Gelatin".POLYMERS 14.18(2022):16.

入库方式： OAI收割

来源：过程工程研究所

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