Freeze-thaw-assisted aqueous two-phase system as a green and low-cost option for analytical grade B-phycoerythrin production from unicellular microalgae Porphyridium purpureum
文献类型：期刊论文
| 作者 | Ji, Liang1; Liu, Yueling1; Luo, Jianquan2; Fan, Jianhua1,3,4,5 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2022-09-01 |
| 卷号 | 67页码:12 |
| ISSN号 | 2211-9264 |
| 关键词 | Microalgae Porphyridium purpureum Phycoerythrin Freeze-thaw Aqueous two-phase system |
| DOI | 10.1016/j.algal.2022.102831 |
| 英文摘要 | Microalgae are promising alternative sources for natural phycobiliprotein production since they grow rapidly and can be cultured on a large scale. However, the overall economic viability of analytical grade phycoerythrin production from microalgae is limited by the high cost of the pretreatment required to break down the cell wall, as well as the expensive reagents and equipment used during purification. This study developed a novel extraction and purification process to recovery B-phycoerythrin (B-PE) in a freeze-thaw-assisted aqueous two-phase system (FT-ATPS) from Porphyridium purpureum. The effects of culture strategy, extraction method and purification factors on B-PE production were studied systematically. The results revealed that low light induced B-PE synthesis, whereas hindered the growth. The stepwise light supplement strategy alleviated the growth limitation under low-light conditions and increased the final B-PE production, up to 182.1 +/- 4.1 mg/L (increasing by 73.1 %). Compared with the traditional electricity highly-consumed ultrasonication method, the green freeze-thaw method could extract B-PE without damaging the cell structure and save much more power (> 98 %), and the extraction rate reached the highest when frozen at -80 C. The purity and concentration of B -PE in crude extract were proved to have a significant effect on ATPS purification, and the higher the former, the better the latter. A total production cost of 0.045 $/mg (decreasing by 96.2 %) and purity of 5.6 +/- 0.2 (A(545)/A(280)) for analytical grade B-PE production were achieved by FT-ATPS based on 4 % polyethylene glycol (PEG2,000) and 20 % ammonium sulfate. Relative to the conventional method of phycoerythrin production, the method developed herein has greater safety, lower cost, and more environmental benefits, which can be function appropriately in the expensive purification of analytical grade phycobiliproteins. |
| WOS关键词 | R-PHYCOERYTHRIN ; SPIRULINA-PLATENSIS ; C-PHYCOCYANIN ; PURIFICATION ; EXTRACTION ; EFFICIENT ; RECOVERY ; CULTIVATION ; MACROALGAE ; SEPARATION |
| 资助项目 | Natural Science Foundation of Shanghai[21ZR1416400] ; Natural Science Foundation of Shandong Province[ZR2019ZD17] ; National Key Research and Development Project of China[2019YFA0906300] ; National Key Research and Development Project of China[2020YFA0907304] ; State Key Laboratory of Bioreactor Engineering |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000857786900003 |
| 资助机构 | Natural Science Foundation of Shanghai ; Natural Science Foundation of Shandong Province ; National Key Research and Development Project of China ; State Key Laboratory of Bioreactor Engineering |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/54722]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Fan, Jianhua |
| 作者单位 | 1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China 4.Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China 5.130 Meilong Rd, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Liang,Liu, Yueling,Luo, Jianquan,et al. Freeze-thaw-assisted aqueous two-phase system as a green and low-cost option for analytical grade B-phycoerythrin production from unicellular microalgae Porphyridium purpureum[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,67:12. |
| APA | Ji, Liang,Liu, Yueling,Luo, Jianquan,&Fan, Jianhua.(2022).Freeze-thaw-assisted aqueous two-phase system as a green and low-cost option for analytical grade B-phycoerythrin production from unicellular microalgae Porphyridium purpureum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,67,12. |
| MLA | Ji, Liang,et al."Freeze-thaw-assisted aqueous two-phase system as a green and low-cost option for analytical grade B-phycoerythrin production from unicellular microalgae Porphyridium purpureum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 67(2022):12. |
入库方式： OAI收割
来源：过程工程研究所
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