L-Cys-Assisted Conversion of H-2/CO2 to Biochemicals Using Clostridium ljungdahlii
文献类型:期刊论文
作者 | Yang, Yuling1,2; Cao, Weifeng2,3; Shen, Fei2,3; Liu, Zhiqian1; Qin, Linli1; Liang, Xinquan1; Wan, Yinhua2,3 |
刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY |
出版日期 | 2022-10-10 |
页码 | 17 |
ISSN号 | 0273-2289 |
关键词 | Clostridium ljungdahlii Cysteine Acetic Acid 2 3-Butanediol Ethanol |
DOI | 10.1007/s12010-022-04174-2 |
英文摘要 | Carbon fixation and conversion based on Clostridium ljungdahlii have great potential for the sustainable production of biochemicals (i.e., 2,3-butanediol, acetic acid, and ethanol). Here, the effects of reducing agents on the production of biochemicals from H-2/CO2 using C. ljungdahlii were studied. It was found that the element S and reducing power could significantly affect the production of biochemicals, and cysteine (Cys) was better than sodium sulfide for the production of biochemicals, especially for the production of 2,3-butanediol. Moreover, comparing to the control (i.e., without the addition of Cys), the gene expression profiles indicated that the fdh and adhE1 were significantly upregulated with the addition of Cys, which involved in pathways of the CO2 fixation and ethanol production. Therefore, the irreplaceability of Cys on the production of biochemicals was both caused by its utilization as a reducing agent and its effect on the metabolic pathway. Finally, compared to the control, the production of 2,3-butanediol was increased by 2.17 times under the addition of 1.7 g/L Cys. |
WOS关键词 | SYNTHESIS GAS FERMENTATION ; ETHANOL-PRODUCTION ; SYNGAS FERMENTATION ; CARBOXIDIVORANS P7 ; BUTANOL PRODUCTION ; CARBON-MONOXIDE ; AUTOETHANOGENUM ; CELL ; OPTIMIZATION ; BIOFUEL |
资助项目 | Beijing Natural Science Foundation, China[5182025] ; Fundamental Research Funds for the Public Research Institutes of Chinese Academy of Inspection and Quarantine[2020JK004] ; National Natural Science Foundation of China, China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005] |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000865688300002 |
资助机构 | Beijing Natural Science Foundation, China ; Fundamental Research Funds for the Public Research Institutes of Chinese Academy of Inspection and Quarantine ; National Natural Science Foundation of China, China ; National High Technology Research and Development Program of China |
源URL | [http://ir.ipe.ac.cn/handle/122111/55023] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Cao, Weifeng; Liang, Xinquan |
作者单位 | 1.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Yuling,Cao, Weifeng,Shen, Fei,et al. L-Cys-Assisted Conversion of H-2/CO2 to Biochemicals Using Clostridium ljungdahlii[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2022:17. |
APA | Yang, Yuling.,Cao, Weifeng.,Shen, Fei.,Liu, Zhiqian.,Qin, Linli.,...&Wan, Yinhua.(2022).L-Cys-Assisted Conversion of H-2/CO2 to Biochemicals Using Clostridium ljungdahlii.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,17. |
MLA | Yang, Yuling,et al."L-Cys-Assisted Conversion of H-2/CO2 to Biochemicals Using Clostridium ljungdahlii".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022):17. |
入库方式: OAI收割
来源:过程工程研究所
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