Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol
文献类型：期刊论文
| 作者 | Liu, Simin1,2,3; Mi, Jiali1,2,3; Song, Kejing4; Qi, Haishan1,2,3; Zhang, Lei1,2,3 |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2022-10-06 |
| 页码 | 12 |
| 关键词 | Cadaverine De novo synthesis Glycerol Microbial consortium Synthetic biology |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-022-03306-2 |
| 英文摘要 | Objectives 1,5-pentanediamine (cadaverine) is a C5 platform chemical, also an important raw material for bio-polyamide PA5X. With increasing concerns about the depletion of fossil resources and global environmental protection, cadaverine bio-production has attracted more attentions. Results Here, a microbial consortium consisting of Corynebacterium glutamicum cgl-FDK and Escherichia coli BL-ABST-Spy was constructed to de novo synthesize cadaverine utilizing glycerol as the sole carbon resource. The glycerol utilization pathway was initially constructed in C. glutamicum cgl-FDK to produce lysine from glycerol. Then, the pyridoxal 5 '-phosphate (PLP) biosynthesis pathway and SpyTag/SpyCatcher protein-ligation system for lysine decarboxylase (CadA) and cadaverine-lysine antiporter protein (CadB) were introduced into E. coli BL-ABST-Spy to synthesize cadaverine from lysine. Furthermore, the fermentation conditions of microbial consortium were optimized and the cadaverine production reached 9.3 g/L with glycerol as the sole carbon source. Conclusions This work provides a promising strategy for efficiently producing cadaverine from glycerol with an artificial microbial consortium. |
| WOS关键词 | CORYNEBACTERIUM-GLUTAMICUM ; ESCHERICHIA-COLI ; PATHWAY ; STRAIN |
| 资助项目 | National Key R&D Program of China[2018YFA0902200] ; National Natural Science Foundation of China[21606165] ; National Natural Science Foundation of China[21621004] ; National Natural Science Foundation of China[21422605] ; Program of Introducing Talents of Discipline to Universities[BP0618007] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000864597800002 |
| 出版者 | SPRINGER |
| 资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; Program of Introducing Talents of Discipline to Universities |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/55036]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Qi, Haishan |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, 135 Yaguan Rd, Tianjin 300072, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Frontier Sci Ctr Synthet Biol, 135 Yaguan Rd, Tianjin 300350, Peoples R China 3.Tianjin Univ, Key Lab Syst Bioengn MOE, 135 Yaguan Rd, Tianjin 300350, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Simin,Mi, Jiali,Song, Kejing,et al. Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol[J]. BIOTECHNOLOGY LETTERS,2022:12. |
| APA | Liu, Simin,Mi, Jiali,Song, Kejing,Qi, Haishan,&Zhang, Lei.(2022).Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol.BIOTECHNOLOGY LETTERS,12. |
| MLA | Liu, Simin,et al."Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol".BIOTECHNOLOGY LETTERS (2022):12. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。