Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus
文献类型：期刊论文
| 作者 | Chen, Huayou1,2; Li, Shouzhi1; Cui, Zhoulei1; Feng, Tao1; Wang, Hongcheng1; Ni, Zhong1; Gao, Ebin1; Fang, Zhen1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2022-10-17 |
| 页码 | 17 |
| 关键词 | Lignin Manganese peroxidase Irpex lacteus Degradation Tween-80 Oxalic acid |
| ISSN号 | 0273-2289 |
| DOI | 10.1007/s12010-022-04189-9 |
| 英文摘要 | Lignocellulose in maize straw includes cellulose, hemicellulose, and lignin, and the degradation of lignocellulose is a complex process in which multiple enzymes are jointly involved. In exploring the co-degradation of a certain substrate by multiple enzymes, different enzymes are combined freely for the achievement of the effective synergism. Additionally, some organic acids and small molecule aromatic compounds can also increase the enzymatic activity of lignin enzymes and improve the degradation rate of lignin. In this study, manganese peroxidase (MnP) from Irpex lacteus (I. lacteus) was heterologously expressed in food-grade Schizosaccharomyces pombe (S. pombe). The multiple enzymes co-fermentation conditions were initially screened by orthogonal tests: 0.5% CaCl2, 1% 10,000 U/g Laccase (Lac), 0.3% MnSO4, and 0.4% glucose oxidase (GOD). It was showed that the lignin degradation rate could reach 65.85% after 3 days of synergistic degradation with the addition of 0.02% Tween-80, 0.5 mM oxalic acid. This indicates that oxalic acid has a promoting effect on the activity of MnP, and the promoting effect is more significant when Tween-80 is complexed with oxalic acid. |
| WOS关键词 | NONPHENOLIC LIGNIN ; LACCASE ; WOOD ; DEPOLYMERIZATION ; DIGESTIBILITY ; QUALITY |
| 资助项目 | national key research and development program[2021YFC2103004] ; national key research and development program[2021YFA0910402] ; Open Funding Project of the State Key Laboratory of Biochemical Engineering, China[2018KF-02] ; Key Research and Development Program (Social Development) of Zhenjiang City[SH2020021] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000868964100001 |
| 出版者 | SPRINGER |
| 资助机构 | national key research and development program ; Open Funding Project of the State Key Laboratory of Biochemical Engineering, China ; Key Research and Development Program (Social Development) of Zhenjiang City |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/55145]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Chen, Huayou; Wang, Hongcheng |
| 作者单位 | 1.Jiangsu Univ, Sch Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 10090, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Huayou,Li, Shouzhi,Cui, Zhoulei,et al. Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2022:17. |
| APA | Chen, Huayou.,Li, Shouzhi.,Cui, Zhoulei.,Feng, Tao.,Wang, Hongcheng.,...&Fang, Zhen.(2022).Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,17. |
| MLA | Chen, Huayou,et al."Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022):17. |
入库方式： OAI收割
来源：过程工程研究所
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