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Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution

文献类型：期刊论文

作者Ren, Xiaokang1,2; Zhao, Luyang1; Yuan, Chengqian1; Shi, Mengqian1; Xing, Ruirui1,2; Yan, Xuehai1,2,3
刊名CHEMICAL ENGINEERING JOURNAL
出版日期2022-12-15
卷号450页码:8
关键词Peptide Tyrosine Enzymatic oxygenation Chemical evolution Pathway selectivity
ISSN号1385-8947
DOI10.1016/j.cej.2022.138293
英文摘要The pathway selectivity of chemical evolution controlled by enzymes, with proteins and peptides as substrates, is of critical significance to determine biological processes and functions. In nature, organisms utilize tyrosinase to catalyse oxidation reactions of tyrosine to produce multifunctional compounds in different pathways. However, it remains a great challenge to manipulate the pathway selection of tyrosinase-guided chemical evolution for achieving biomaterials with on-demand functions. We herein report that controlling oxygen concentration is effective in regulating the evolution of tyrosinase-catalysed oxygenation pathways of tyrosine-containing peptides and even proteins. The melanin formation pathway predominates under oxygen-enriched conditions, while fluorescent compounds are preferentially generated under hypoxic conditions. The chemical evolution of tyrosine-containing peptides, regardless of their neighbouring amino acid types and sequences, tends to be governed by these two competitive pathways. These competitive pathways are manipulated by conformational transformation of tyrosinase active centre that is determined by oxygen concentration. Moreover, the oxygen-controlled chemical evolution of tyrosine-containing biomolecules can be broadly realized by tyrosinase to obtain melanin-based photothermal materials or multicolour fluorescent materials. This work reveals the plausible role of oxygen in the pathway selectivity of enzyme-controlled chemical evolution of tyrosine-containing peptides and verifies the possibility of fluorescent detection in living cultured melanoma cells, which provides a versatile strategy for precise design and engineering of tyrosine-involved multifunctional biomaterials.
WOS关键词MELANIN ; RED
资助项目National Science Fund for Distinguished Young Scholars of China[22025207] ; Innovation Research Community Science Fund of China[21821005] ; National Natural Science Foundation of China[22072154] ; National Natural Science Foundation of China[22077122] ; National Natural Science Foundation of Hebei Province[B2020103025] ; National Natural Science Foundation of Hebei Province[B2020103036] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[2021048]
WOS研究方向Engineering
语种英语
WOS记录号WOS:000875249700005
出版者ELSEVIER SCIENCE SA
资助机构National Science Fund for Distinguished Young Scholars of China ; Innovation Research Community Science Fund of China ; National Natural Science Foundation of China ; National Natural Science Foundation of Hebei Province ; Youth Innovation Promotion Association of Chinese Academy of Sciences
源URL[http://ir.ipe.ac.cn/handle/122111/55299]  
专题中国科学院过程工程研究所
通讯作者Xing, Ruirui; Yan, Xuehai
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 North Second St, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Ctr Mesosci, 1 North Second St, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Ren, Xiaokang,Zhao, Luyang,Yuan, Chengqian,et al. Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution[J]. CHEMICAL ENGINEERING JOURNAL,2022,450:8.
APA Ren, Xiaokang,Zhao, Luyang,Yuan, Chengqian,Shi, Mengqian,Xing, Ruirui,&Yan, Xuehai.(2022).Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution.CHEMICAL ENGINEERING JOURNAL,450,8.
MLA Ren, Xiaokang,et al."Enzyme-driven oxygen-fuelled pathway selectivity of tyrosine-containing peptide oxidation evolution".CHEMICAL ENGINEERING JOURNAL 450(2022):8.

入库方式： OAI收割

来源：过程工程研究所

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