Integrated microsphere-packed bed enzymatic membrane reactor for enhanced bioconversion efficiency and stability: A proof-of-concept study
文献类型：期刊论文
| 作者 | Zhang, Hao1; Liu, Luke1,2; Pinelo, Manuel3; Huang, Yuhong1,2; Zhou, Weiqing1; Wan, Yinhua1,2; Luo, Jianquan1,2 |
| 刊名 | JOURNAL OF MEMBRANE SCIENCE
 |
| 出版日期 | 2022-09-15 |
| 卷号 | 658页码:10 |
| ISSN号 | 0376-7388 |
| 关键词 | Enzymatic membrane reactor Microspheres Enzyme immobilization Membrane fouling Oligodextran |
| DOI | 10.1016/j.memsci.2022.120732 |
| 英文摘要 | Fabricating high-performance enzyme reactors is requested for achieving efficient and stable bioconversions, but remains challenging, because few of them can possess high enzyme loading, sufficient mixing, and efficient mass transfer at the same time. Herein, we propose to develop a novel enzymatic packed bed membrane reactor (EPBMR) by integrating the advantages of both packed bed reactor (PBR) and enzymatic membrane reactor (EMR). A prototype study is conducted with the simplified enzyme-loaded microsphere-ultrafiltration EMR model (Mic-UF EMR). Invertase and dextranase are used in this work to produce glucose and oligodextran by hydrolysis of sucrose and dextran, respectively. Specifically, the use of microspheres can enlarge the contact area between enzymes and substrates and mitigate membrane fouling induced by free enzymes. Thus, Free&Mic-UF EMR (with both free and immobilized enzymes) exhibits a higher sucrose conversion rate (84%) than the EMR with free invertase (34%) and a negligible decline in sucrose conversion for 36 h continuous operation. Membrane fouling is ameliorated by alkaline cleaning and implementation of covalent bonding strategy. In addition, commercial resins with larger sizes are employed to replace konjac glucomannan microspheres (KGM) which reduce the pressure drop of EMR. Finally, by selecting the UF membrane with proper molecular weight cut-off (MWCO), the dextranase-based Mic-UF EMR system successfully produces oligodextran with desired molecular weight (Mw) and narrow Mw distribution. The outcome of this work not only offers a novel enzyme reactor construction strategy but also provides guidance for regulating the performance of EMR. |
| WOS关键词 | IMMOBILIZATION ; HYDROLYSIS ; FILTRATION ; LACTULOSE |
| 资助项目 | Tianjin Synthetic Biotech-nology Innovation Capacity Improvement Project[TSBICIP-KJGG-003] |
| WOS研究方向 | Engineering ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000886256300005 |
| 资助机构 | Tianjin Synthetic Biotech-nology Innovation Capacity Improvement Project |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/55601]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhou, Weiqing; Luo, Jianquan |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China 3.Tech Univ Denmark, Proc & Syst Engn Ctr, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark |
| 推荐引用方式 GB/T 7714 | Zhang, Hao,Liu, Luke,Pinelo, Manuel,et al. Integrated microsphere-packed bed enzymatic membrane reactor for enhanced bioconversion efficiency and stability: A proof-of-concept study[J]. JOURNAL OF MEMBRANE SCIENCE,2022,658:10. |
| APA | Zhang, Hao.,Liu, Luke.,Pinelo, Manuel.,Huang, Yuhong.,Zhou, Weiqing.,...&Luo, Jianquan.(2022).Integrated microsphere-packed bed enzymatic membrane reactor for enhanced bioconversion efficiency and stability: A proof-of-concept study.JOURNAL OF MEMBRANE SCIENCE,658,10. |
| MLA | Zhang, Hao,et al."Integrated microsphere-packed bed enzymatic membrane reactor for enhanced bioconversion efficiency and stability: A proof-of-concept study".JOURNAL OF MEMBRANE SCIENCE 658(2022):10. |
入库方式： OAI收割
来源：过程工程研究所
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