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Conjugation with 8-arm PEG and CRM(197 )enhances the immunogenicity of SARS-CoV-2 ORF8 protein

文献类型：期刊论文

作者Tang, Xiaozhao1,2; Yu, Weili2; Shen, Lijuan2; Qi, Jinming2; Hu, Tao2
刊名INTERNATIONAL IMMUNOPHARMACOLOGY
出版日期2022-08-01
卷号109页码:8
ISSN号1567-5769
关键词SARS-CoV-2 ORF8 CRM197 eight-arm PEG Vaccine
DOI10.1016/j.intimp.2022.108922
英文摘要Safe and effective vaccines are urgently needed to combat the COVID-19 pandemic. However, the SARS-CoV-2 variants raise concerns about the effectiveness of vaccines. As a SARS-CoV-2 antigen target, ORF8 strongly inhibits the IFN-13 and NF-kappa B-responsive promoter, and can be potentially used for the development of SARS-CoV-2 vaccine. However, it is necessary to improve the immunogenicity of ORF8 by adjuvants or delivery systems. CRM197 was a carrier protein with the ability to activate T helper cells for antigens. Eight-arm PEG could conjugate multiple antigen molecules in one entity with inherent adjuvant effect. In the present study, ORF8 was conjugated with CRM197 and 8-arm PEG, respectively. The cellular and humoral immune responses to the conjugates (ORF8-CRM and ORF8-PEG) were evaluated in the BALB/c mice. As compared with ORF8-CRM and ORF8 administrated with aluminum adjuvant (ORF8/AL), ORF8-PEG induced a higher ORF8-specific IgG titer (2.6 x 104), higher levels of cytokines (IFN-gamma, TNF-alpha, IFN-13, and IL-5), stronger splenocyte proliferation. Thus, conjugation with 8-arm PEG was an effective method to improve the immune response to ORF8. Moreover, ORF8-PEG did not lead to apparent toxicity to the cardiac, liver and renal functions. ORF8-PEG was expected to act as an effective vaccine to provide the immune protection against SARS-CoV-2.
资助项目Beijing Natural Science Foundation[M21013] ; National Natural Science Foundation of China[31970875] ; National Key Research and Development Project of China[2018YFA0900804]
WOS研究方向Immunology ; Pharmacology & Pharmacy
语种英语
出版者ELSEVIER
WOS记录号WOS:000886030800007
资助机构Beijing Natural Science Foundation ; National Natural Science Foundation of China ; National Key Research and Development Project of China
源URL[http://ir.ipe.ac.cn/handle/122111/55623]  
专题中国科学院过程工程研究所
通讯作者Qi, Jinming; Hu, Tao
作者单位1.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Xiaozhao,Yu, Weili,Shen, Lijuan,et al. Conjugation with 8-arm PEG and CRM(197 )enhances the immunogenicity of SARS-CoV-2 ORF8 protein[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2022,109:8.
APA Tang, Xiaozhao,Yu, Weili,Shen, Lijuan,Qi, Jinming,&Hu, Tao.(2022).Conjugation with 8-arm PEG and CRM(197 )enhances the immunogenicity of SARS-CoV-2 ORF8 protein.INTERNATIONAL IMMUNOPHARMACOLOGY,109,8.
MLA Tang, Xiaozhao,et al."Conjugation with 8-arm PEG and CRM(197 )enhances the immunogenicity of SARS-CoV-2 ORF8 protein".INTERNATIONAL IMMUNOPHARMACOLOGY 109(2022):8.

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来源：过程工程研究所

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