A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink
文献类型：期刊论文
| 作者 | Xu, Jie1; Yang, Shuangjia1; Su, Ya1; Hu, Xueyan1; Xi, Yue1; Cheng, Yuen Yee2; Kang, Yue3; Nie, Yi4,5; Pan, Bo6; Song, Kedong1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOPRINTING
 |
| 出版日期 | 2023 |
| 卷号 | 9期号:1页码:109-130 |
| ISSN号 | 2424-7723 |
| 关键词 | Tumor model Decellularized extracellular matrix Gelatin Sodium alginate Three-dimensional bioprinting |
| DOI | 10.18063/ijb.v9i1.630 |
| 英文摘要 | Tissue-engineered scaffolds are more commonly used to construct three-dimension-al (3D) tumor models for in vitro studies when compared to the conventional two-dimensional (2D) cell culture because the microenvironments provided by the 3D tumor models closely resemble the in vivo system and could achieve higher success rate when the scaffolds are translated for use in pre-clinical animal model. Physical properties, heterogeneity, and cell behaviors of the model could be regulated to simu-late different tumors by changing the components and concentrations of materials. In this study, a novel 3D breast tumor model was fabricated by bioprinting using a bioink that consists of porcine liver-derived decellularized extracellular matrix (dECM) with different concentrations of gelatin and sodium alginate. Primary cells were removed while extracellular matrix components of porcine liver were preserved. The rheolog-ical properties of biomimetic bioinks and the physical properties of hybrid scaffolds were investigated, and we found that the addition of gelatin increased hydrophilia and viscoelasticity, while the addition of alginate increased mechanical properties and porosity. The swelling ratio, compression modulus, and porosity could reach 835.43 +/- 130.61%, 9.64 +/- 0.41 kPa, and 76.62 +/- 4.43%, respectively. L929 cells and the mouse breast tumor cells 4T1 were subsequently inoculated to evaluate biocompatibility of the scaffolds and to form the 3D models. The results showed that all scaffolds exhibited good biocompatibility, and the average diameter of tumor spheres could reach 148.52 +/- 8.02 mu m on 7 d. These findings suggest that the 3D breast tumor model could serve as an effective platform for anticancer drug screening and cancer research in vitro. |
| WOS关键词 | IN-VITRO ; ACID HYDROGEL ; HYBRID SCAFFOLD ; PORCINE LIVER ; TISSUE ; CANCER ; CULTURE ; OPTIMIZATION |
| 资助项目 | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Fundamental Research Funds for the Central Universities ; [31670978] ; [132027] ; [KF1111] ; [DUT21YG113/DUT22YG213/DUT22YG116] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| 出版者 | WHIOCE PUBL PTE LTD |
| WOS记录号 | WOS:000879518600002 |
| 资助机构 | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/55718]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Kang, Yue; Nie, Yi; Pan, Bo; Song, Kedong |
| 作者单位 | 1.Dalian Univ Technol, Dalian R&D Ctr Stem Cell & Tissue Engn, State Key Lab Fine Chem, Dalian 116024, Peoples R China 2.Univ Technol Sydney, Fac Sci, Inst Biomed Mat & Devices, Sydney, NSW 2007, Australia 3.China Med Univ, Dept Breast Surg, Canc Hosp, 44 Xiaoheyan Rd, Shenyang 110042, Peoples R China 4.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 6.Dalian Med Univ, Dept Breast Surg, Hosp 2, 467 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jie,Yang, Shuangjia,Su, Ya,et al. A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink[J]. INTERNATIONAL JOURNAL OF BIOPRINTING,2023,9(1):109-130. |
| APA | Xu, Jie.,Yang, Shuangjia.,Su, Ya.,Hu, Xueyan.,Xi, Yue.,...&Song, Kedong.(2023).A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink.INTERNATIONAL JOURNAL OF BIOPRINTING,9(1),109-130. |
| MLA | Xu, Jie,et al."A 3D bioprinted tumor model fabricated with gelatin/sodium alginate/decellularized extracellular matrix bioink".INTERNATIONAL JOURNAL OF BIOPRINTING 9.1(2023):109-130. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。