Study on ionic liquids based novel method for separation and purification of silkworm pupa protein
文献类型：期刊论文
| 作者 | Zhang, Wen1,2,4; Yang, Shaoqi1,3; Ren, Baozeng4; Lu, Xingmei1; Jia, Cai1 |
| 刊名 | CHINESE CHEMICAL LETTERS
 |
| 出版日期 | 2022 |
| 卷号 | 34期号:1页码:6 |
| 关键词 | Silkworm pupa protein Ionic liquids Protein backbone Silkworm pupa Separation |
| ISSN号 | 1001-8417 |
| DOI | 10.1016/j.cclet.2022.04.072 |
| 英文摘要 | Silkworm pupa protein (SPP) that obtained by traditional method usually had a high fat content, which would impose restrictions on the further use of SPP. Herein, various functionalized ionic liquids (ILs) were used to extract SPP from silkworm pupae, the structure-performance relationship of ILs with their SPP separation performance were explored at the same time. The research showed that the maxi-mum extraction yield of SPP was up to 62.6% with less than 0.5% low fat content by using 1-ethyl-3-methylimidazolium chloride ([Emim]Cl), when the dissolution experiment was conducted at 90 ? for 24 h with ethanol bath as the regeneration solvent. Comparing with the structure of raw material, the regenerated SPP maintained the native protein backbone. Meanwhile, all regenerated SPP showed a de-creased crystallinity, which also exhibited decreased fraction of the alpha-helix comparing to that beta-sheet united with coil random structures. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. |
| WOS关键词 | WOOL KERATIN ; CELLULOSE ; DISSOLUTION ; EXTRACTION |
| 资助项目 | National Natural Science Foundation of China[81673400] ; National Natural Science Foundation of China[21878292] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000893634100025 |
| 出版者 | ELSEVIER SCIENCE INC |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/56136]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Jia, Cai |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Beijing Key Lab Ion Liquids Clean Proc, CAS Key Lab Green Proc & Engn,State Key Lab Multip, Beijing 100190, Peoples R China 2.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 3.Henan Agr Univ, Coll Mech & Elect Engn, Key Lab New Mat & Facil Rural Renewable Energy, Minist Agr & Rural Affairs, Zhengzhou 450002, Peoples R China 4.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wen,Yang, Shaoqi,Ren, Baozeng,et al. Study on ionic liquids based novel method for separation and purification of silkworm pupa protein[J]. CHINESE CHEMICAL LETTERS,2022,34(1):6. |
| APA | Zhang, Wen,Yang, Shaoqi,Ren, Baozeng,Lu, Xingmei,&Jia, Cai.(2022).Study on ionic liquids based novel method for separation and purification of silkworm pupa protein.CHINESE CHEMICAL LETTERS,34(1),6. |
| MLA | Zhang, Wen,et al."Study on ionic liquids based novel method for separation and purification of silkworm pupa protein".CHINESE CHEMICAL LETTERS 34.1(2022):6. |
入库方式： OAI收割
来源：过程工程研究所
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