Mechanism and inhibition of abnormal chromatographic behavior of serotype type a inactivated foot and mouth disease virus in high-performance size-exclusion chromatography
文献类型：期刊论文
| 作者 | Yang, Yanli1; Li, Ming1; Zhao, Yi1; Lin, Xuan2; Su, Zhiguo2; Xin, Fei1; Du, Xiaojie1; Zheng, Kan1; Han, Rui1; Pan, Yiping1 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2022-12-20 |
| 卷号 | 1686页码:10 |
| ISSN号 | 0021-9673 |
| 关键词 | HPSEC Foot and mouth disease virus Non-specific adsorption Chaotropic salt Amino acid |
| DOI | 10.1016/j.chroma.2022.463648 |
| 英文摘要 | High-performance size-exclusion chromatography (HPSEC) has been developed for the rapid and quanti-tative analysis of inactivated foot and mouth disease virus (FMDV) and adopted by regulatory agencies and vaccine manufacturers. However, strong non-specific adsorption of type A/AKT III FMDV was found on some batches of TSK G40 0 0 SWXL column, which significantly affected the analysis accuracy. The ad-sorption mechanism was studied by investigating the charge and hydrophobicity of A/AKT III FMDV and another serotype O/Mya 98, as well as several model proteins, by zeta potential and hydrophobic inter-action chromatography analysis. Adsorption was related to both the FMDV strain and column lots. Some specific amino acids residues on the A/AKT III FMDV surface may strongly interact with the column if the silica-based stationary phase was not completely diol-modified. Several amino acids and chaotropic salts were screened as additives in the mobile phase to suppress the non-specific adsorption of AKT III FMDV in HPSEC analysis. Results showed that adding 0.4 M of arginine (Arg), lysine (Lys), NaClO4, or NaSCN achieved 100% FMDV recovery and normal retention time. Suppression of interaction between FMDV and the backbone of the silica matrix through competitive binding with residues of FMDV or the matrix is considered as the main mechanism by which these four additives act as suppressors. The addition of Arg, NaClO4, or NaSCN led to an apparent decrease in the thermal dissociation temperature Tm of FMDV, whereas Lys slightly increased viral stability. Finally, the mobile phase comprising 0.4 M Lys was screened as optimum that allowed accurate quantification of both two serotypes of FMDV according to method val-idation; particularly, a relative standard deviation (RSD) < 5% was achieved for AKT III FMDV using three different lots of columns. (c) 2022 Elsevier B.V. All rights reserved. |
| WOS关键词 | QUALITY-CONTROL ; MOBILE-PHASE ; ARGININE ; VACCINE ; PARTICLES ; HPLC ; STABILITY ; PROVIDES ; ANTIGEN ; SALTS |
| 资助项目 | National Key R & D Program of China ; National Natural Science Founda-tion of China ; [2021YFC2103402] ; [31970872] ; [21821005] ; [21973097] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000904553300004 |
| 资助机构 | National Key R & D Program of China ; National Natural Science Founda-tion of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/56346]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | He, Sun; Zhang, Songping |
| 作者单位 | 1.TECON Pharmaceut Suzhou Co Ltd, Suzhou 215000, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yanli,Li, Ming,Zhao, Yi,et al. Mechanism and inhibition of abnormal chromatographic behavior of serotype type a inactivated foot and mouth disease virus in high-performance size-exclusion chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2022,1686:10. |
| APA | Yang, Yanli.,Li, Ming.,Zhao, Yi.,Lin, Xuan.,Su, Zhiguo.,...&Zhang, Songping.(2022).Mechanism and inhibition of abnormal chromatographic behavior of serotype type a inactivated foot and mouth disease virus in high-performance size-exclusion chromatography.JOURNAL OF CHROMATOGRAPHY A,1686,10. |
| MLA | Yang, Yanli,et al."Mechanism and inhibition of abnormal chromatographic behavior of serotype type a inactivated foot and mouth disease virus in high-performance size-exclusion chromatography".JOURNAL OF CHROMATOGRAPHY A 1686(2022):10. |
入库方式： OAI收割
来源：过程工程研究所
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