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Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

文献类型：期刊论文

作者Cao, Fengqiang1,2,3; Ming, Yali3,4; Gao, Weixiang3,4; Ge, Jia3; Ogino, Kenji1,2
刊名JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
出版日期2022-09-01
期号187页码:18
ISSN号1940-087X
DOI10.3791/64406
英文摘要The cellular affinity of micro-/nanoparticles is the precondition for cellular recognition, cellular uptake, and activation, which are essential for drug delivery and immune response. The present study stemmed from the observation that the effects of charge, size, and shape of solid particles on cell affinity are usually considered, but we seldom realize the essential role of softness, dynamic restructuring phenomenon, and complex interface interaction in cellular affinity. Here, we developed poly-lactic-co-glycolic acid (PLGA) nanoparticle-stabilized Pickering emulsion (PNPE) that overcame the shortcomings of rigid forms and simulated the flexibility and fluidity of pathogens. A method was set up to test the affinity of PNPE to cell surfaces and elaborate on the subsequent internalization by immune cells. The affinity of PNPE to bio-mimetic extracellular vesicles (bEVs)-the replacement for bone marrow dendritic cells (BMDCs)-was determined using a quartz crystal microbalance with dissipation monitoring (QCM-D), which allowed real-time monitoring of cell-emulsion adhesion. Subsequently, the PNPE was used to deliver the antigen (ovalbumin, OVA) and the uptake of the antigens by BMDCs was observed using confocal laser scanning microscope (CLSM). Representative results showed that the PNPE immediately decreased frequency (Delta F) when it encountered the bEVs, indicating rapid adhesion and high affinity of the PNPE to the BMDCs. PNPE showed significantly stronger binding to the cell membrane than PLGA microparticles (PMPs) and AddaVax adjuvant (denoted as surfactant-stabilized nano-emulsion [SSE]). Furthermore, owing to the enhanced cellular affinity to the immunocytes through dynamic curvature changes and lateral diffusions, antigen uptake was subsequently boosted compared with PMPs and SSE. This protocol provides insights for designing novel formulations with high cell affinity and efficient antigen internalization, providing a platform for the development of efficient vaccines.
WOS关键词DENDRITIC CELLS ; IMMUNE-RESPONSE ; MEMBRANE-FUSION ; DRUG-DELIVERY ; BINDING ; VIRUS ; SIZE
资助项目National Key Research and Development Program of China[2021YFE020527] ; National Key Research and Development Program of China[2021YFC2302605] ; National Key Research and Development Program of China[2021YFC2300142] ; Basic Frontier Scientific Research Program of Chinese Academy of Sciences[ZDBS-LY-SLH040] ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China[21821005]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000909465300041
出版者JOURNAL OF VISUALIZED EXPERIMENTS
资助机构National Key Research and Development Program of China ; Basic Frontier Scientific Research Program of Chinese Academy of Sciences ; Foundation for Innovative Research Groups of the National Natural Science Foundation of China
源URL[http://ir.ipe.ac.cn/handle/122111/56463]  
专题中国科学院过程工程研究所
通讯作者Ogino, Kenji
作者单位1.Tokyo Univ Agr & Technol, Grad Sch Bio Applicat & Syst Engn, Tokyo, Japan
2.Tokyo Univ Agr & Technol, Inst Global Innovat Res, Tokyo, Japan
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Cao, Fengqiang,Ming, Yali,Gao, Weixiang,et al. Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2022(187):18.
APA Cao, Fengqiang,Ming, Yali,Gao, Weixiang,Ge, Jia,&Ogino, Kenji.(2022).Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS(187),18.
MLA Cao, Fengqiang,et al."Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS .187(2022):18.

入库方式： OAI收割

来源：过程工程研究所

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