Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1
文献类型：期刊论文
| 作者 | Cao, Weifeng1,2; Deng, Tingting3; Shen, Fei1,2; Rong, Fan1,2; Qiao, Changsheng4; Wan, Yinhua1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2022-12-31 |
| 卷号 | 223页码:722-731 |
| ISSN号 | 0141-8130 |
| 关键词 | Poly(beta-L-malic acid) Aureobasidium melanogenum Electrolytic stimulation Bioelectric-fermentation system Oxidation-reduction potential |
| DOI | 10.1016/j.ijbiomac.2022.11.026 |
| 英文摘要 | Poly (beta-L-malic acid) (PMLA) is attracting industrial interest for its potential application in medicine and other industries. In this study, electrolytic stimulation assisted PMLA production was developed. Firstly, it was found that the pentavalent nitrogen source (i.e., NO3-) was more suitable for PMLA production. Secondly, a usual single-chamber bioelectric-fermentation system (BES) cannot improve PMLA production, which can only promote cell growth. Then, a new single-chamber BES with an external circulation was developed, where the PMLA metabolism was further intensified. Finally, the integration of NO3- addition and electrolytic stimulation mode (c) showed a positive synergy on the PMLA production. Compared to the case without NO3- addition and electrolytic stimulation, the PMLA production was increased by 22.9 % using the integrated process. Moreover, compared to the case without the electrolytic stimulation mode (c), it was revealed that the different genes involved in 12 metabolic subsystems using the integrated process, where 31 and 177 genes were up-regulated and down-regulated, respectively. The up-regulated genes were mainly participated in melanin metabolic process, catalase activity, and oxidoreductase activity. Hence, the integration of electrolytic stimulation represents a novel approach to improve PMLA production. |
| WOS关键词 | CORYNEBACTERIUM-GLUTAMICUM ; MICROBIAL ELECTROSYNTHESIS ; ANTIOXIDANT ACTIVITY ; NEUTRAL RED ; PULLULANS ; BIOSYNTHESIS ; FERMENTATION ; MELANIN |
| 资助项目 | National Natural Science Foundation of China, China ; National Key R & D Program of China, China ; Beijing Natural Science Foundation, China ; [22278412] ; [2021YFF0601903] ; [8202044] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000906838700004 |
| 资助机构 | National Natural Science Foundation of China, China ; National Key R & D Program of China, China ; Beijing Natural Science Foundation, China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/56479]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Cao, Weifeng; Wan, Yinhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China 3.Chinese Acad Inspection & Quarantine, Agroprod Safety Res Ctr, Beijing 100123, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Weifeng,Deng, Tingting,Shen, Fei,et al. Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,223:722-731. |
| APA | Cao, Weifeng,Deng, Tingting,Shen, Fei,Rong, Fan,Qiao, Changsheng,&Wan, Yinhua.(2022).Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,223,722-731. |
| MLA | Cao, Weifeng,et al."Electrolytic stimulation in aid of poly(beta-L-malic acid) production by Aureobasidium melanogenum ipe-1".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 223(2022):722-731. |
入库方式： OAI收割
来源：过程工程研究所
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