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Porous inert material as promising carrier enhanced cellulase production from Trichoderma reesei in solid-state fermentation

文献类型：期刊论文

作者Wang, Lan1,2; Lin, Xuecheng1,2; Zhou, Yaoyao1,2; Chen, Hongzhang1,2
刊名PROCESS BIOCHEMISTRY
出版日期2022-11-01
卷号122页码:316-322
关键词Cellulase Polyurethane carbon foams Inert carrier Solid-state fermentation Repeated cycle fermentation
ISSN号1359-5113
DOI10.1016/j.procbio.2022.10.007
英文摘要Developing a promising fermentation strategy with high cellulase yield and stable fermenting performance is an imperative way to overcome the cellulase costs limitation. In this study, a novel solid-state fermentation (SSF) strategy using polyurethane carbon foams (PAF) as inert carriers was developed to improve cellulase production by Trichoderma reesei (T. reesei). The effects of significant process parameters such as pore size and thickness of carrier on cellulase production were evaluated. Repeated cycle fermentation using inert carrier for cellulase production was assessed by measuring cellulase activity. Results indicated that inert carrier was conducive to cellulase production and the higher cellulase activity of 0.368 FPU/mL was achieved in SSF with PAF rather than PPF or PUF. More cellulase components could be obtained from SSF with inert carrier compared with submerged fermentation. In addition, higher cellulase activity (0.393 FPU/mL) could be achieved at 0.6 mm pore and 15 mm thickness of PAF. The repeated cycle fermentation experiments showed that maximum cellulase activity of 0.514 FPU/mL was obtained over 6 cycles and the fermentation performance was still stable in SSF with PAF. Therefore, SSF using inert carrier is simple, low cost, and environmentally benign, making it an attractive method for cellulase production.
WOS关键词PENICILLIUM-OXALICUM ; SUGARCANE BAGASSE ; ETHANOL ; ENZYMES ; BIOCONVERSION
资助项目National Key Research and Development Program of China[2019YFB1503800] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences[XFJ-STS-QYZX-123]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
语种英语
WOS记录号WOS:000911593300003
出版者ELSEVIER SCI LTD
资助机构National Key Research and Development Program of China ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences
源URL[http://ir.ipe.ac.cn/handle/122111/56491]  
专题中国科学院过程工程研究所
通讯作者Wang, Lan
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Beijing Key Lab Biomass Refining Engn, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Lan,Lin, Xuecheng,Zhou, Yaoyao,et al. Porous inert material as promising carrier enhanced cellulase production from Trichoderma reesei in solid-state fermentation[J]. PROCESS BIOCHEMISTRY,2022,122:316-322.
APA Wang, Lan,Lin, Xuecheng,Zhou, Yaoyao,&Chen, Hongzhang.(2022).Porous inert material as promising carrier enhanced cellulase production from Trichoderma reesei in solid-state fermentation.PROCESS BIOCHEMISTRY,122,316-322.
MLA Wang, Lan,et al."Porous inert material as promising carrier enhanced cellulase production from Trichoderma reesei in solid-state fermentation".PROCESS BIOCHEMISTRY 122(2022):316-322.

入库方式： OAI收割

来源：过程工程研究所

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