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Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice

文献类型：期刊论文

作者Wang, Yan2,3; Yang, Lvyu2,3; Zhou, Hansheng1; Zhang, Kunlin3; Zhao, Mei2,3
刊名FRONTIERS IN GENETICS
出版日期2023-01-19
卷号13页码:13
关键词high-throughput sequencing microRNA gene expression addiction regulatory network L-methionine cocaine
DOI10.3389/fgene.2022.1076156
通讯作者Zhao, Mei(zhaomei@psych.ac.cn)
英文摘要Background and Aims: Methionine has been proven to inhibit addictive behaviors of cocaine dependence. This study aimed to identify the potential mechanisms of MET relating to its inhibitory effects on cocaine induced cellular and behavioral changes. Methods: MRNA and miRNA high-throughput sequencing of the prefrontal cortex in a mouse model of cocaine conditioned place preference (CPP) combined with L-methionine was performed. Differentially expressed miRNAs (DE-miRNAs) and differentially expressed genes (DEGs) regulated by cocaine and inhibited by L-methionine were identified. DEGs were mapped to STRING database to construct a protein-protein interaction (PPI) network. Then, the identified DEGs were subjected to the DAVID webserver for functional annotation. Finally, miRNA-mRNA regulatory network and miRNA-mRNA-TF regulatory networks were established to screen key DE-miRNAs and coregulation network in Cytoscape. Results: Sequencing data analysis showed that L-methionine reversely regulated genes and miRNAs affected by cocaine. Pathways associated with drug addiction only enriched in CS-down with MC-up genes targeted by DE-miRNAs including GABAergic synapse, Glutamatergic synapse, Circadian entrainment, Axon guidance and Calcium signaling pathway. Drug addiction associated network was formed of 22 DEGs including calcium channel (Cacna1c, Cacna1e, Cacna1g and Cacng8), ephrin receptor genes (Ephb6 and Epha8) and ryanodine receptor genes (Ryr1 and Ryr2). Calcium channel gene network were identified as a core gene network modulated by L-methionine in response to cocaine dependence. Moreover, it was predicted that Grin1 and Fosb presented in TF-miRNA-mRNA coregulation network with a high degree of interaction as hub genes and interacted calcium channels. Conclusion: These identified key genes, miRNA and coregulation network demonstrated the efficacy of L-methionine in counteracting the effects of cocaine CPP. To a certain degree, it may provide some hints to better understand the underlying mechanism on L-methionine in response to cocaine abuse.
收录类别SCI
WOS关键词DNA METHYLATION ; CALCIUM-CHANNEL ; EXPRESSION ; PROTEIN ; ADDICTION ; RECEPTOR ; BRAIN
资助项目National Natural Science Foundation of China[61401459] ; National Natural Science Foundation of China[91132728] ; National Natural Science Foundation of China[31741062] ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
WOS研究方向Genetics & Heredity
语种英语
WOS记录号WOS:000926078800001
出版者FRONTIERS MEDIA SA
资助机构National Natural Science Foundation of China ; Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
源URL[http://ir.psych.ac.cn/handle/311026/44747]  
专题心理研究所_中国科学院心理健康重点实验室
通讯作者Zhao, Mei
作者单位1.Linyi Peoples Hosp, Dept Pharm, Linyi, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Dept psychol, Beijing, Peoples R China
3.Inst Psychol, CAS Key Lab Mental Hlth, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yan,Yang, Lvyu,Zhou, Hansheng,et al. Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice[J]. FRONTIERS IN GENETICS,2023,13:13.
APA Wang, Yan,Yang, Lvyu,Zhou, Hansheng,Zhang, Kunlin,&Zhao, Mei.(2023).Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice.FRONTIERS IN GENETICS,13,13.
MLA Wang, Yan,et al."Identification of miRNA-mediated gene regulatory networks in L-methionine exposure counteracts cocaine-conditioned place preference in mice".FRONTIERS IN GENETICS 13(2023):13.

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来源：心理研究所

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