Antibiotic Toxicity Profiles of Escherichia coli Strains Lacking DNA Methyltransferases
文献类型：期刊论文
| 作者 | Chen, Zheng; Wang, Hailin |
| 刊名 | ACS OMEGA
 |
| 出版日期 | 2021-03-23 |
| 卷号 | 6期号:11页码:7834-7840 |
| ISSN号 | 2470-1343 |
| 英文摘要 | Antibiotic-resistant bacteria are causing more antibiotic treatment failures. Developing new antibiotics and identifying bacterial targets will help to mitigate the emergence and reduce the spread of antibiotic resistance in the environment. We investigated whether DNA methyltransferase (MTase can be an adjunct target for improving antibiotic toxicity. We used Escherichia coli as an example. The genes encoding DNA adenine MTase and cytosine MTase, dam and dcm, respectively, were separately knocked out using the lambda Red system in E. coli MG1655. MG1655 and the two knockout strains were separately exposed in 96-well plates to 20 antibiotics from five classes. The EC50 values of almost all of the tested antibiotics were lower in the dam and dcm knockout lines than that of the control. Our statistical analysis showed that the variations observed in EC50 values were independent of the mechanism underlying each antibiotic's mechanistic action. |
| WOS研究方向 | Chemistry, Multidisciplinary |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45660]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zheng,Wang, Hailin. Antibiotic Toxicity Profiles of Escherichia coli Strains Lacking DNA Methyltransferases[J]. ACS OMEGA,2021,6(11):7834-7840. |
| APA | Chen, Zheng,&Wang, Hailin.(2021).Antibiotic Toxicity Profiles of Escherichia coli Strains Lacking DNA Methyltransferases.ACS OMEGA,6(11),7834-7840. |
| MLA | Chen, Zheng,et al."Antibiotic Toxicity Profiles of Escherichia coli Strains Lacking DNA Methyltransferases".ACS OMEGA 6.11(2021):7834-7840. |
入库方式： OAI收割
来源：生态环境研究中心
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