Chirality of gold nanocluster affects its interaction with coagulation factor XII
文献类型：期刊论文
| 作者 | Hao, Fang ; Geng, Fanglan; Zhao, Xingchen; Liu, Rui; Liu, Qian S.; Zhou, Qunfang; Jiang, Guibin
|
| 刊名 | NANOIMPACT
 |
| 出版日期 | 2021-04 |
| 卷号 | 22页码:- |
| 关键词 | Gold nanoclusters Chirality Coagulation factor XII Protein corona Nanobiological effect |
| ISSN号 | 2452-0748 |
| 英文摘要 | Probing the interaction of nanomaterials (NMs) with proteins is the basic step for biological safety assessment. Many physiochemical factors of NMs play important roles in binding with proteins as they determine the binding process. Among them, the chirality-related biological effects and nanotoxicology have not been fully understood. As NMs are mainly exposed to human circulatory system with intentional or unintentional exposure, understanding the interaction mechanism of plasma functional proteins with chiral NMs is of great importance. Herein, we show the interaction of chiral gold nanoclusters (AuNCs), L- and D-cysteine coated AuNC (i.e., L-AuNC and DAuNC, respectively) with human coagulation factor XII (FXII, an important plasma zymogen initiating the inner coagulation system). D-AuNC exhibited weak binding affinity for FXII, induced FXII aggregation due to significant conformational change, which then activated the FXII for further cleavage. In contrast to D-AuNC, the binding affinity of L-AuNC for FXII was strong and their bioconjugate was quite stable without aggregation. LAuNC induced the structural change and autoactivation of FXII to a lower extent. Moreover, the enzymatic activity of FXIIa (the activated form of FXII) was influenced upon incubation with L- AuNCs and D-AuNCs with different molecular mechanisms. The finding will expand the understanding of the nanobiological effects of chiral NMs and suggest the potential application in nanomedicine. |
| WOS研究方向 | Environmental Sciences ; Nanoscience & Nanotechnology |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45684]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310000, Peoples R China 4.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, Fang,Geng, Fanglan,Zhao, Xingchen,et al. Chirality of gold nanocluster affects its interaction with coagulation factor XII[J]. NANOIMPACT,2021,22:-. |
| APA | Hao, Fang.,Geng, Fanglan.,Zhao, Xingchen.,Liu, Rui.,Liu, Qian S..,...&Jiang, Guibin.(2021).Chirality of gold nanocluster affects its interaction with coagulation factor XII.NANOIMPACT,22,-. |
| MLA | Hao, Fang,et al."Chirality of gold nanocluster affects its interaction with coagulation factor XII".NANOIMPACT 22(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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