CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
文献类型：期刊论文
| 作者 | Feng, Wei; Newbigging, Ashley M.; Tao, Jeffrey; Cao, Yiren; Peng, Hanyong; Le, Connie; Wu, Jinjun; Pang, Bo; Li, Juan; Tyrrell, D. Lorne |
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2021-04-07 |
| 卷号 | 12期号:13页码:- |
| ISSN号 | 2041-6520 |
| 英文摘要 | Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) protein systems have transformed the field of genome editing and transcriptional modulation. Progress in CRISPR-Cas technology has also advanced molecular detection of diverse targets, ranging from nucleic acids to proteins. Incorporating CRISPR-Cas systems with various nucleic acid amplification strategies enables the generation of amplified detection signals, enrichment of low-abundance molecular targets, improvements in analytical specificity and sensitivity, and development of point-of-care (POC) diagnostic techniques. These systems take advantage of various Cas proteins for their particular features, including RNA-guided endonuclease activity, sequence-specific recognition, multiple turnover trans-cleavage activity of Cas12 and Cas13, and unwinding and nicking ability of Cas9. Integrating a CRISPR-Cas system after nucleic acid amplification improves detection specificity due to RNA-guided recognition of specific sequences of amplicons. Incorporating CRISPR-Cas before nucleic acid amplification enables enrichment of rare and low-abundance nucleic acid targets and depletion of unwanted abundant nucleic acids. Unwinding of dsDNA to ssDNA using CRISPR-Cas9 at a moderate temperature facilitates techniques for achieving isothermal exponential amplification of nucleic acids. A combination of CRISPR-Cas systems with functional nucleic acids (FNAs) and molecular translators enables the detection of non-nucleic acid targets, such as proteins, metal ions, and small molecules. Successful integrations of CRISPR technology with nucleic acid amplification techniques result in highly sensitive and rapid detection of SARS-CoV-2, the virus that causes the COVID-19 pandemic. |
| WOS研究方向 | Chemistry, Multidisciplinary |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45696]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Jilin Univ, Sch Publ Hlth, 1163 Xinmin St, Changchun 130021, Jilin, Peoples R China 2.Univ Alberta, Fac Med & Dent, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada 3.Univ Alberta, Li Ka Shing Inst Virol, Dept Med Microbiol & Immunol, Fac Med & Dent, Edmonton, AB T6G 2E1, Canada 4.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Wei,Newbigging, Ashley M.,Tao, Jeffrey,et al. CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules[J]. CHEMICAL SCIENCE,2021,12(13):-. |
| APA | Feng, Wei.,Newbigging, Ashley M..,Tao, Jeffrey.,Cao, Yiren.,Peng, Hanyong.,...&Le, X. Chris.(2021).CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules.CHEMICAL SCIENCE,12(13),-. |
| MLA | Feng, Wei,et al."CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules".CHEMICAL SCIENCE 12.13(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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