Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system
文献类型：期刊论文
| 作者 | Zhu, Nuanfei; Li, Xuesong; Liu, Ye; Liu, Jingfu; Wang, Yawei; Wu, Xiangyang; Zhang, Zhen |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2021-08-25 |
| 卷号 | 784页码:- |
| 关键词 | Immunoassay Antibody Emerging organic pollutants DNA tetrahedron G-quadruplex hemin DNAzyme Environmental Dibutyl phthalate |
| ISSN号 | 0048-9697 |
| 英文摘要 | Dibutyl phthalate (DBP) is considered as one of the most widely used phthalate esters (PAEs), which has attracted worldwide concerns because of its potential threats to eco-environments and human health. Systematic investigations of DBP environmental occurrence contribute to the further risk assessment, which depends on effective and available analytical methods. In this study, an amplified ratiometric fluorescence ELISA was established for sensitive and high-throughput detection of DBP in the aquatic system based on a novel tetrahedral DNA nanostructure (TDN)-scaffolded-DNAzyme (Tetrazyme). Wherein, Tetrazyme was prepared by the precise folding of G-quadruplex sequence on three vertex angles of the TDN, together with hemin as the horseradish peroxidase (HRP)-mimicking enzyme. The rigid TDN avoided the local overcrowding effect to provide a reasonable spatial spacing on the interface for G-quadruplex sequence, increasing the collision chance between DNAzyme and substrates, improving the catalytic ability of DNAzyme effectively. Besides, streptavidin (SA) and biotin (bio) were used to anchor TDN and antibody, in which the specific binding of SA/bio could make more Tetrazyme conjugate on each signal element, resulting in the dual signal amplification. Meanwhile, the accuracy and precision were enhanced owing to the inherent built-in rectification to the environment from the dual output ratiometric fluorescence assay. Under the optimized conditions, the detection limit of this proposed method was 0.17 ng/mL (16 times lower than that of conventional ELISA using the same antibody) with a satisfactory accuracy (recoveries, 79.0%-116.2%; CV, 2.1-6.5%). Overall, this platform provides a promising way for accurate, sensitive and rapid determination of DBP from environmental waters. (c) 2021 Elsevier B.V. All rights reserved. |
| WOS研究方向 | Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45709]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China 2.Zhenjiang Environm Monitoring Ctr, Zhenjiang 212013, Jiangsu, Peoples R China 3.Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples |
| 推荐引用方式 GB/T 7714 | Zhu, Nuanfei,Li, Xuesong,Liu, Ye,et al. Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,784:-. |
| APA | Zhu, Nuanfei.,Li, Xuesong.,Liu, Ye.,Liu, Jingfu.,Wang, Yawei.,...&Zhang, Zhen.(2021).Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system.SCIENCE OF THE TOTAL ENVIRONMENT,784,-. |
| MLA | Zhu, Nuanfei,et al."Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system".SCIENCE OF THE TOTAL ENVIRONMENT 784(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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