Expression and Biochemical Characterization of a Novel Marine Chitosanase from Streptomyces niveus Suitable for Preparation of Chitobiose
文献类型：期刊论文
| 作者 | Chen, Tong; Cheng, Gong; Jiao, Siming; Ren, Lishi; Zhao, Chuanfang; Wei, Jinhua; Han, Juntian; Pei, Meishan; Du, Yuguang; Li, Jian-Jun |
| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2021-06 |
| 卷号 | 19期号:6页码:- |
| 关键词 | endo-chitosanases Streptomyces niveus pH and temperature-rate profile pH and thermal stability chitobiose partially acetylated chitooligosaccharide structure modelling |
| 英文摘要 | It is known that bioactivities of chitooligosaccharide (COS) are closely related to the degree of polymerization (DP); therefore, it is essential to prepare COS with controllable DP, such as chitobiose showing high antioxidant and antihyperlipidemia activities. In this study, BLAST, sequence alignment and phylogenetic analysis of characterized glycoside hydrolase (GH) 46 endo-chitosanases revealed that a chitosanase Sn1-CSN from Streptomyces niveus was different from others. Sn1-CSN was overexpressed in E. coli, purified and characterized in detail. It showed the highest activity at pH 6.0 and exhibited superior stability between pH 4.0 and pH 11.0. Sn1-CSN displayed the highest activity at 50 degrees C and was fairly stable at <= 45 degrees C. Its apparent kinetic parameters against chitosan (DDA: degree of deacetylation, >94%) were determined, with K-m and k(cat) values of 1.8 mg/mL and 88.3 s(-1), respectively. Cu2+ enhanced the activity of Sn1-CSN by 54.2%, whereas Fe3+ inhibited activity by 15.1%. Hydrolysis products of chitosan (DDA > 94%) by Sn1-CSN were mainly composed of chitobiose (87.3%), whereas partially acetylated chitosan with DDA 69% was mainly converted into partially acetylated COS with DP 2-13. This endo-chitosanase has great potential to be used for the preparation of chitobiose and partially acetylated COS with different DPs. |
| WOS研究方向 | Chemistry, Medicinal ; Pharmacology & Pharmacy |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45728]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Jinan Univ, Sch Chem & Chem Engn, Jinan 250022, Peoples R China 2.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, Natl Engn Res Ctr Biotechnol Beijing, Natl Key Lab Biochem Engn,Inst Proc En 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Tong,Cheng, Gong,Jiao, Siming,et al. Expression and Biochemical Characterization of a Novel Marine Chitosanase from Streptomyces niveus Suitable for Preparation of Chitobiose[J]. MARINE DRUGS,2021,19(6):-. |
| APA | Chen, Tong.,Cheng, Gong.,Jiao, Siming.,Ren, Lishi.,Zhao, Chuanfang.,...&Li, Jian-Jun.(2021).Expression and Biochemical Characterization of a Novel Marine Chitosanase from Streptomyces niveus Suitable for Preparation of Chitobiose.MARINE DRUGS,19(6),-. |
| MLA | Chen, Tong,et al."Expression and Biochemical Characterization of a Novel Marine Chitosanase from Streptomyces niveus Suitable for Preparation of Chitobiose".MARINE DRUGS 19.6(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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