Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2
文献类型：期刊论文
| 作者 | Ku, Tingting; Hao, Fang ; Yang, Xiaoxi; Rao, Ziyu; Liu, Qian S.; Sang, Nan; Faiola, Francesco; Zhou, Qunfang; Jiang, Guibin
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| 出版日期 | 2021-03-02 |
| 卷号 | 55期号:5页码:3144-3155 |
| ISSN号 | 0013-936X |
| 英文摘要 | The tremendous potential for graphene quantum dots (GQDs) in biomedical applications has led to growing concerns of their health risks in human beings. However, present studies mainly focused on oxidative stress, apoptosis, and other general toxicity effects; the knowledge on the developmental toxicity and the related regulatory mechanisms is still far from sufficient. Our study revealed the development retardation of mouse embryonic stem cells (mESCs) caused by GQDs with a novel DNA methylation epigenetic mechanism. Specifically, GQDs were internalized into cells mainly via energy-dependent endocytosis, and a significant fraction of internalized GQDs remained in the cells even after a 48-h clearance period. Albeit with unobservable cytotoxicity or any influences on cell pluripotency, significant retardation was found in the in vitro differentiation of the mESCs into embryoid bodies (EBs) with the upregulation of Sox2 levels in GQD pretreatment groups. Importantly, this effect could be contributed by GQD-induced inhibition in CpG methylation of Sox2 through altering methyltransferase and demethyltransferase transcriptional expressions, and the demethyltransferase inhibitor, bobcat339 hydrochloride, reduced GQD-induced upregulation of Sox2. The current study first demonstrated that GQDs compromised the differentiation program of the mESCs, potentially causing development retardation. Exposure to this nanomaterial during gestation or early developmental period would cause adverse health risks and is worthy of more attention. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45737]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Univ Chinese Acad Sci, Sch Environm, Hangzhou Inst Adv Study, Beijing 100049, Peoples R China 4.Shanxi Univ, Coll Environm & Resource, Res Ctr Environm & Hlth, Taiyuan 030006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ku, Tingting,Hao, Fang,Yang, Xiaoxi,et al. Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2021,55(5):3144-3155. |
| APA | Ku, Tingting.,Hao, Fang.,Yang, Xiaoxi.,Rao, Ziyu.,Liu, Qian S..,...&Jiang, Guibin.(2021).Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(5),3144-3155. |
| MLA | Ku, Tingting,et al."Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.5(2021):3144-3155. |
入库方式： OAI收割
来源：生态环境研究中心
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