Identification and interaction mechanism of protein corona on silver nanoparticles with different sizes and the cellular responses
文献类型：期刊论文
| 作者 | Zhao, Zongshan; Li, Guoliang; Liu, Qian S.; Liu, Wei; Qu, Guangbo; Hu, Ligang; Long, Yanmin; Cai, Zongwei; Zhao, Xingchen; Jiang, Guibin
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| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2021-07-15 |
| 卷号 | 414页码:- |
| 关键词 | Dose metric Curvature Silver nanoparticle Human serum albumin Cytotoxicity |
| ISSN号 | 0304-3894 |
| 英文摘要 | With the potential biomedical applications of nanomaterials such as silver nanoparticles (SNPs), nanotoxicity concerns are growing, and the importance of NP and protein interactions is far from being addressed enough. Here, we identified the major binding protein on SNPs in blood as human serum albumin (HSA) using polyacrylamide gel electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry. By comparing with the previous methods, we emphasized surface area concentration as a new dose metric to address the importance of NP curvature. SNPs interacted with cysteine and cystine, disrupting the secondary structure and conformation of HSA, and this tendency became stronger on small SNPs than large ones. The protein corona significantly alleviated the toxicity and decreased SNPs' internalization in a particle size-dependent manner, where more significant inhibition effects occurred on larger particles at the same area concentration. These findings may shed light on nanotoxicity and also the design of safe nanomaterials by a comprehensive preconsideration of the metrological method. |
| WOS研究方向 | Engineering, Environmental ; Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45745]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Qingdao Univ, Coll Environm Sci & Engn, Qingdao 266071, Peoples R China 2.Chinese Acad Inspect & Quarantine, Inst Chem Safety, Beijing 100124, Peoples R China 3.Jianghan Univ, Inst Environm & Hlth, Hubei Key Lab Environm & Hlth Effects Persistent, Wuhan 430056, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 5.Hong Kong Baptist Univ, Dept Chem, State Key Lab Environm & Biol Anal, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zongshan,Li, Guoliang,Liu, Qian S.,et al. Identification and interaction mechanism of protein corona on silver nanoparticles with different sizes and the cellular responses[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,414:-. |
| APA | Zhao, Zongshan.,Li, Guoliang.,Liu, Qian S..,Liu, Wei.,Qu, Guangbo.,...&Jiang, Guibin.(2021).Identification and interaction mechanism of protein corona on silver nanoparticles with different sizes and the cellular responses.JOURNAL OF HAZARDOUS MATERIALS,414,-. |
| MLA | Zhao, Zongshan,et al."Identification and interaction mechanism of protein corona on silver nanoparticles with different sizes and the cellular responses".JOURNAL OF HAZARDOUS MATERIALS 414(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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