Identification of estrogen receptor target genes involved in gonadal feminization caused by estrogen in Xenopus laevis
文献类型：期刊论文
| 作者 | Li, Yuanyuan; Li, Jinbo ; Shen, Yanping; Xiong, Yiming; Li, Xinghong; Qin, Zhanfen
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| 刊名 | AQUATIC TOXICOLOGY
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| 出版日期 | 2021-03 |
| 卷号 | 232页码:- |
| 关键词 | Estrogenic endocrine-disrupting chemicals Estrogen receptor Target gene Xenopus laevis Gonadal feminization Chromatin immunoprecipitation |
| ISSN号 | 0166-445X |
| 英文摘要 | Estrogens and estrogenic endocrine disrupting chemicals can cause gonadal feminization in some vertebrates mainly through estrogen receptor (ER), but the underlying molecular mechanisms are unclear. The present study aimed to identify ER target genes involved in estrogen-caused gonadal feminization in Xenopus laevis. Based on our recent transcriptomic data that 10 nM 17 beta-estradiol (E2) altered gene transcription in feminizing gonads of male X. laevis at NF stages 48, 50, and 52, we searched estrogen response element (ERE) using the Dragon ERE Finder software in the promoter region of all the E2-regulated genes. As a result, 163 genes containing ERE sequence were identified as predicted ER target genes at NF stage 50 (on the 14th day postfertilization), a crucial stage for gonadal feminization. Then, some of these predicted ER target genes were further investigated, mainly including the genes that were suggested to be involved in E2-caused gonadal feminization and genes being dramatically up or down-regulated by E2. Fifteen genes were demonstrated to be responsive to E2, in turn ER antagonist blocked the E2-regulated transcription. Finally, we identified 10 genes that can bind to ERa by a chromatin immunoprecipitation-qPCR. Taken together, we identified the 10 genes that contain predicted ERE sequences, are responsive to estrogen and ER antagonist, and have ability to bind to ER as ER target genes, including pglyrp2, apoa1, fgb, tdo2, ca6, nags, cpb2, tmprss6, nudc, zwiIch. Our results could help to improve the understanding of the molecular mechanisms for gonadal feminization caused by estrogenic endocrine disrupting chemicals in X. laevis, and even in other species. |
| WOS研究方向 | Marine & Freshwater Biology ; Toxicology |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45748]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuanyuan,Li, Jinbo,Shen, Yanping,et al. Identification of estrogen receptor target genes involved in gonadal feminization caused by estrogen in Xenopus laevis[J]. AQUATIC TOXICOLOGY,2021,232:-. |
| APA | Li, Yuanyuan,Li, Jinbo,Shen, Yanping,Xiong, Yiming,Li, Xinghong,&Qin, Zhanfen.(2021).Identification of estrogen receptor target genes involved in gonadal feminization caused by estrogen in Xenopus laevis.AQUATIC TOXICOLOGY,232,-. |
| MLA | Li, Yuanyuan,et al."Identification of estrogen receptor target genes involved in gonadal feminization caused by estrogen in Xenopus laevis".AQUATIC TOXICOLOGY 232(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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