Immunoassay of Small Molecule Mediated by a Triply Functional DNA
文献类型：期刊论文
| 作者 | Li, Yapiao; Yu, Fangzhi; Chen, Shaokun; Zhang, Ning; Wang, Hailin; Zhao, Qiang |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2021-03-23 |
| 卷号 | 93期号:11页码:4794-4799 |
| ISSN号 | 0003-2700 |
| 英文摘要 | Benefiting from specific target recognition by antibodies, the immunoassay is one of the widely used assays for the detection of biologically and environmentally important small molecules in broad fields. It can be challenge to isolate small molecules from their antibody complex in an immobilization-free immunoassay with separation for the detection of small-molecule targets. Here we present an immunoassay mediated by a triply functional DNA probe. A DNA strand is dually labeled with a fluorophore and the target small molecule. This DNA probe integrates three functions, including specific binding to the antibody, signal reporting for sensitive fluorescence detection, and carrying negative charges to facilitate capillary electrophoresis (CE) separation. The binding of the probe to an antibody brings many negative charges in the complex and causes significant changes in mass-to-charge ratios, so the antibody-probe complex can be well separated from the unbound probe in CE analysis. A simple immunoassay is achieved by target competition with this DNA probe for antibody binding in CE coupled to ultrasensitive laser-induced fluorescence (LIF) detection. To show a proof of concept, we detected two model small-molecule targets, digoxin, a therapeutic drug, and ochratoxin A (OTA), an important mycotoxin for food safety. In addition, the use of two DNA probes with distinguished migration times in CE allowed the simultaneous detection of OTA and digoxin. This immunoassay provides new opportunities for wide applications. |
| WOS研究方向 | Chemistry, Analytical |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45751]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yapiao,Yu, Fangzhi,Chen, Shaokun,et al. Immunoassay of Small Molecule Mediated by a Triply Functional DNA[J]. ANALYTICAL CHEMISTRY,2021,93(11):4794-4799. |
| APA | Li, Yapiao,Yu, Fangzhi,Chen, Shaokun,Zhang, Ning,Wang, Hailin,&Zhao, Qiang.(2021).Immunoassay of Small Molecule Mediated by a Triply Functional DNA.ANALYTICAL CHEMISTRY,93(11),4794-4799. |
| MLA | Li, Yapiao,et al."Immunoassay of Small Molecule Mediated by a Triply Functional DNA".ANALYTICAL CHEMISTRY 93.11(2021):4794-4799. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。