Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry
文献类型：期刊论文
| 作者 | Li, Yanshen; Chen, Anqi; Mao, Xin; Sun, Mingxue; Yang, Shupeng; Li, Juan; You, Yanli; Wu, Yongning; Jiang, Guibin
|
| 刊名 | FOOD CHEMISTRY
 |
| 出版日期 | 2021-02-01 |
| 卷号 | 337页码:- |
| 关键词 | Multiple immunoaffinity columns Cereals Deoxynivalenol T-2 Toxin UHPLC-MS/MS |
| ISSN号 | 0308-8146 |
| 英文摘要 | Deoxynivalenol (DON) and T-2 toxin are major trichothecenes contaminated in cereals, which might bring harmful effects to humans. In this research, mixed anti-DON and anti-T-2 mAb were used for multiple immunoaffinity columns (mIACs) preparation. Under the optimal conditions, column capacities were tested at 1280 ng/mL for DON and 1160 ng/mL for T-2 toxin. Regeneration investigation showed mIACs capacities were over 510 ng/mL for DON and 440 ng/mL for T-2 toxin in 10 recycle usages. Good performances were obtained when applying mIACs purification coupled UHPLC-MS/MS for spiked samples with limit of detection at 3-13 mu g/kg and mean recoveries at 79.0-97.6%. Applying to estimate the exposure of DON and T-2 toxin in commercial samples, maize samples were 100% DON positive and rice samples were 40% DON positive while T-2 toxin was negative in all tested samples. The proposed method is reliable and suitable for monitoring DON and T-2 toxin in cereal samples. |
| WOS研究方向 | Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45787]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Yantai Univ, Coll Life Sci, Yantai 264005, Shandong, Peoples R China 3.Chinese Acad Agr Sci, Inst Apicultural Res, Beijing 100093, Peoples R China 4.Univ Western Ontario, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada 5.Chinese Acad Med Sci, China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Res Unit 20 |
| 推荐引用方式 GB/T 7714 | Li, Yanshen,Chen, Anqi,Mao, Xin,et al. Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry[J]. FOOD CHEMISTRY,2021,337:-. |
| APA | Li, Yanshen.,Chen, Anqi.,Mao, Xin.,Sun, Mingxue.,Yang, Shupeng.,...&Jiang, Guibin.(2021).Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry.FOOD CHEMISTRY,337,-. |
| MLA | Li, Yanshen,et al."Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry".FOOD CHEMISTRY 337(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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