One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution
文献类型：期刊论文
| 作者 | Ma, Yangde; Zhang, Ning; Chen, Shaokun; Sun, Jingwei; Liu, Yan; Li, Xiangjun; Wang, Hailin |
| 刊名 | ANALYST
 |
| 出版日期 | 2021-02-07 |
| 卷号 | 146期号:3页码:820-824 |
| ISSN号 | 0003-2654 |
| 英文摘要 | Here we establish a one-pot reaction to directly convert the DNA base 5-hydroxymethylcytosine (5hmC) to an intramolecular cyclization nucleobase, which loses both protons of the exocyclic N4-amino group and thus is recognized as thymine (T) by DNA polymerase. Based on this 5hmC-specific reaction, a prospective bisulfite-free strategy for 5hmC sequencing is proposed. This is also the first example to show modified DNA labeling in non-water solvent-dominant media for DNA sequencing. |
| WOS研究方向 | Chemistry, Analytical |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45801]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Jianghan Univ, Inst Environm & Hlth, 8 Sanjiaohu Rd, Wuhan 430056, Hubei, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, 18 Shuangqing Rd, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Yangde,Zhang, Ning,Chen, Shaokun,et al. One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution[J]. ANALYST,2021,146(3):820-824. |
| APA | Ma, Yangde.,Zhang, Ning.,Chen, Shaokun.,Sun, Jingwei.,Liu, Yan.,...&Wang, Hailin.(2021).One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution.ANALYST,146(3),820-824. |
| MLA | Ma, Yangde,et al."One-pot intramolecular cyclization of 5-hydroxymethylcytosine for sequencing DNA hydroxymethylation at single-base resolution".ANALYST 146.3(2021):820-824. |
入库方式： OAI收割
来源：生态环境研究中心
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