Potent oxidation of DNA by Ru(ii) tri(polypyridyl) complexes under visible light irradiation via a singlet oxygen-mediated mechanism
文献类型：期刊论文
| 作者 | Shao, Jie; Yan, Zhu-Ying; Tang, Miao; Huang, Chun-Hua; Sheng, Zhi-Guo; Chen, Jing; Shao, Bo; Zhu, Ben-Zhan |
| 刊名 | INORGANIC CHEMISTRY FRONTIERS
 |
| 出版日期 | 2021-07-21 |
| 卷号 | 8期号:14页码:3421-+ |
| ISSN号 | 2052-1553 |
| 英文摘要 | Since Ru(ii) tri(polypyridyl) complexes can bind DNA in the groove, the chemistry of metallo-groove-binders has been extended to many applications such as artificial photonucleases and DNA recognizers. Here we found that the major product was N-(2-deoxy-beta-d-erythro-pentofuranosyl)-spiroiminodihydantoin (dSp) when the nucleoside 2 '-deoxyguanosine (dG) was exposed to the visible light irradiation of Ru(ii) tri(polypyridyl) complexes including Ru(bpy)(3)(2+) (bpy = 2,2 '-bipyridyl), Ru(OP)(3)(2+) (OP = 1,10-phenanthroline) and Ru(DIP)(3)(2+) (DIP = 4,7-diphenyl-1,10-phenanthroline). Interestingly, we found that in the oxidation of dsDNA the major oxidation product is 8-oxo-7,8-dihydro-2 '-deoxyguanosine (8-oxodG) instead of dSp. The formation of dSp and 8-oxodG increased significantly in D2O and decreased markedly with the addition of NaN3. [O-18]-Labeled 8-oxodG was also found to be produced more than non-labeled 8-oxodG under an O-18(2) atmosphere. The above results clearly indicated that O-1(2) was the major oxidant during the oxidation of both dG and dsDNA by the irradiation of Ru(ii) complexes, which was further confirmed by both ESR spin-trapping and UV methods. Among the above three Ru(ii) complexes, Ru(DIP)(3)(2+) is the most efficient photosensitized generator of dG oxidation products, while in the oxidation of dsDNA, Ru(bpy)(3)(2+) is the most effective. This is the first report that the irradiation of Ru(ii) polypridyl complexes with visible light can induce potent oxidation of dG and ctDNA to the more mutagenic product dSp and 8-oxodG via an O-1(2)-mediated mechanism. These findings should provide new perspectives for future investigations on whether ubiquitous Ru(ii) complexes could cause oxidatively generated DNA damage in cellular models. |
| WOS研究方向 | Chemistry, Inorganic & Nuclear |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45819]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100085, Peoples R China 2.Res Ctr Ecoenvironm Sci, Joint Inst Environm Sci, Hong Kong, Peoples R China 3.Jining Med Univ, Sch Publ Hlth, Jining 272067, Shandong, Peoples R China 4.Hong Kong Baptist Univ, Hong Kong, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, Jie,Yan, Zhu-Ying,Tang, Miao,et al. Potent oxidation of DNA by Ru(ii) tri(polypyridyl) complexes under visible light irradiation via a singlet oxygen-mediated mechanism[J]. INORGANIC CHEMISTRY FRONTIERS,2021,8(14):3421-+. |
| APA | Shao, Jie.,Yan, Zhu-Ying.,Tang, Miao.,Huang, Chun-Hua.,Sheng, Zhi-Guo.,...&Zhu, Ben-Zhan.(2021).Potent oxidation of DNA by Ru(ii) tri(polypyridyl) complexes under visible light irradiation via a singlet oxygen-mediated mechanism.INORGANIC CHEMISTRY FRONTIERS,8(14),3421-+. |
| MLA | Shao, Jie,et al."Potent oxidation of DNA by Ru(ii) tri(polypyridyl) complexes under visible light irradiation via a singlet oxygen-mediated mechanism".INORGANIC CHEMISTRY FRONTIERS 8.14(2021):3421-+. |
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来源：生态环境研究中心
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