Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA
文献类型：期刊论文
| 作者 | Yuan, Zheng; Zhang, Dapeng ; Yu, Fangzhi; Ma, Yangde; Liu, Yan; Li, Xiangjun; Wang, Hailin
|
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2021-02-14 |
| 卷号 | 12期号:6页码:2039-2049 |
| ISSN号 | 2041-6520 |
| 英文摘要 | Multiple DNA-interacting protein molecules are often dynamically distributed and/or assembled along a DNA molecule to adapt to their intricate functions temporally. However, analytical technology for measuring such binding behaviours is still missing. Here, we demonstrate the unique capacity of a supernuclease for a highly efficient cutting of the unprotected-DNA segments and with complete preservation of the protein-occluded DNA segments at near single-nucleotide resolution. By exploring this high-resolution cutting, an unprecedented assay that allows a precise sequencing of single protected-DNA fragment molecules (SPDFMS) was developed. As relevant applications, relevant information was gained on the respective distribution/assembly patterns and coordinated displacement of single-stranded DNA-binding protein and recombinase RecA, two model proteins, on DNA. Benefiting from this assay, we also for the first time provide direct measurement of the length of single RecA nucleofilaments, showing the predominant stoichiometry of 5-7 RecA monomers per RecA nucleofilament under physiologically relevant conditions. This innovative assay appears as a promising analytical tool for studying diverse protein-DNA interactions implicated in DNA replication, transcription, recombination, repair, and gene editing. |
| WOS研究方向 | Chemistry, Multidisciplinary |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45820]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.UCAS, Inst Adv Study, Inst Environm & Hlth, Hangzhou 310000, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 4.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Zheng,Zhang, Dapeng,Yu, Fangzhi,et al. Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA[J]. CHEMICAL SCIENCE,2021,12(6):2039-2049. |
| APA | Yuan, Zheng.,Zhang, Dapeng.,Yu, Fangzhi.,Ma, Yangde.,Liu, Yan.,...&Wang, Hailin.(2021).Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA.CHEMICAL SCIENCE,12(6),2039-2049. |
| MLA | Yuan, Zheng,et al."Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA".CHEMICAL SCIENCE 12.6(2021):2039-2049. |
入库方式： OAI收割
来源：生态环境研究中心
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