Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure
文献类型：期刊论文
| 作者 | Gao, Lu; Liu, Liying; Tian, Yunfei; Yang, Qianfan; Wu, Peng; Fan, Chunhai; Zhao, Qiang; Li, Feng
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| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2021-05-11 |
| 卷号 | 93期号:18页码:7045-7053 |
| ISSN号 | 0003-2700 |
| 英文摘要 | The development of robust analytical tools capable of probing the formation kinetics and thermodynamics of DNA nanostructures is a crucial step toward better understanding and manufacturing of diverse DNA-based materials. Herein, we introduce a real-time fluorescence anisotropy assay and rationally designed DNA reaction termination probes (DRTPs) as a set of new tools for exploring the formation mechanisms of DNA nanostructures. We deployed these tools for probing the formation of a classic tetrahedral DNA nanostructure (TDN) as a model system. Our tools revealed that the formation of TDN was dominated by simultaneous hybridization, whereas its undesired side products were caused mainly through step-wise hybridization. An optimal reaction temperature exists that favors the formation of TDN over side products. With insight into the TDN formation mechanism, we further engineered magnetic DRTPs to achieve single-step purification of TDN, enabling 10-fold improvement in the ratio between the targeted TDN and undesired side products without tedious procedures or bulky instruments. Combining the optimal reaction and purification conditions, we finally demonstrated the one-pot synthesis and purification of TDN. The analytical techniques offered in this work may hold potential to find wide applications and inspire new analytical methods for structural DNA nanotechnology. |
| WOS研究方向 | Chemistry, Analytical |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45821]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Brock Univ, Ctr Biotechnol, Dept Chem, St Catharines, ON L2S 3A1, Canada 3.Sichuan Univ, Coll Chem, Analyt & Testing Ctr, Key Lab Green Chem & Technol,Minist Educ, Chengdu 610064, Sichuan, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 201240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Lu,Liu, Liying,Tian, Yunfei,et al. Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure[J]. ANALYTICAL CHEMISTRY,2021,93(18):7045-7053. |
| APA | Gao, Lu.,Liu, Liying.,Tian, Yunfei.,Yang, Qianfan.,Wu, Peng.,...&Li, Feng.(2021).Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure.ANALYTICAL CHEMISTRY,93(18),7045-7053. |
| MLA | Gao, Lu,et al."Probing the Formation Kinetics and Thermodynamics with Rationally Designed Analytical Tools Enables One-Pot Synthesis and Purification of a Tetrahedral DNA Nanostructure".ANALYTICAL CHEMISTRY 93.18(2021):7045-7053. |
入库方式： OAI收割
来源：生态环境研究中心
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