Profiling of the assembly of RecA nucleofilaments implies a potential target for environmental factors to disturb DNA repair
文献类型：期刊论文
| 作者 | Yuan, Zheng; Yu, Fangzhi; Zhang, Dapeng ; Wang, Hailin
|
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2021-04 |
| 卷号 | 102页码:283-290 |
| 关键词 | DNA double-strand breaks (DSBs) RecA assembly Homology recombination (HR) ATP hydrolysis DNA damage repair Environmental health |
| ISSN号 | 1001-0742 |
| 英文摘要 | Double-strand breaks (DSBs), one class of the most harmful DNA damage forms that bring elevated health risks, need to be repaired timely and effectively. However, an increasing number of environmental pollutants have been identified to impair DSB repair from various mechanisms. Our previous work indicated that the formation of unsaturated RecA nucleofilaments plays an essential role in homology recombination (HR) pathway which can accurately repair DSBs. In this study, by developing a benzonase cutting protection assay and combining it with traditional electrophoretic mobility shift assay (EMSA) analysis, we further investigated the assembly patterns of four RecA mutants that display differential DSB repair ability and ATPase activity. We observed that the mutants (G204S and S69G) possessing both ATP hydrolysis and DSB repair activities form unsaturated nucleofilaments similar to that formed by the wild type RecA, whereas the other two ATP hydrolysis-deficient mutants (K72R and E96D) that fail to mediate HR form more compacted nucleofilaments in the presence of ATP. These results establish a coupling of ATPase activity and effective DSB repair ability via the assembly status of RecA nucleofilaments. This linkage provides a potential target for environmental factors to disturb the essential HR pathway for DSB repair by suppressing the ATPase activity and altering the assembly pattern of nucleofilaments. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| WOS研究方向 | Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45822]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Jianghan Univ, Inst Environm & Hlth, Wuhan 430056, Peoples R China 2.UCAS, Hangzhou Inst Adv Study, Inst Environm & Hlth, Hangzhou 430056, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Zheng,Yu, Fangzhi,Zhang, Dapeng,et al. Profiling of the assembly of RecA nucleofilaments implies a potential target for environmental factors to disturb DNA repair[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2021,102:283-290. |
| APA | Yuan, Zheng,Yu, Fangzhi,Zhang, Dapeng,&Wang, Hailin.(2021).Profiling of the assembly of RecA nucleofilaments implies a potential target for environmental factors to disturb DNA repair.JOURNAL OF ENVIRONMENTAL SCIENCES,102,283-290. |
| MLA | Yuan, Zheng,et al."Profiling of the assembly of RecA nucleofilaments implies a potential target for environmental factors to disturb DNA repair".JOURNAL OF ENVIRONMENTAL SCIENCES 102(2021):283-290. |
入库方式： OAI收割
来源：生态环境研究中心
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