Simple and rapid determination of dioxin in fish and sea food using a highly sensitive reporter cell line, CBG 2.8D
文献类型：期刊论文
| 作者 | Ding, Gangdou; Wang, Lingyun; Zhang, Songyan; Li, Shuaizhang; Xie, Qunhui; Xu, Li; Zhou, Zhiguang; He, YinFeng; Zhao, Bin |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
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| 出版日期 | 2021-02 |
| 卷号 | 100页码:353-359 |
| 关键词 | Aryl hydrocarbon recepto Reporter gene assay Fish and sea food Dioxins |
| ISSN号 | 1001-0742 |
| 英文摘要 | Food, especially animal origin food is the main source of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and dioxin-like polychlorinated biphenyls (dl -PCBs) for human exposure. So, a simple, rapid and cheap bioassay method is needed for determination of dioxins in food samples. In this study, we used a new highly sensitive reporter cell line to determine the concentration of dioxins in 33 fish and seafood samples. The samples were extracted by shaking with water/isopropanol (1:1 v/v) and hexane and cleaned -up by a multi layered silica gel column and an alumina column, then analyzed using CBG 2.8D cell line. We compared the results obtained from the CBG 2.8D cell assay to those obtained from conventional High -Resolution Gas Chromatography-High Resolution Mass Spectrometry (HRGC-HRMS) analysis. Good correlations were observed between these two methods (r2=0.93). While the slope of regression line was 1.76, the bioanalytical equivalent (BEQ) values were 1.76 folds higher than WHO-TEQ values and the conversion coefficient was 0.568 (the reciprocal of 1.76). In conclusion, CBG 2.8D cell assay was an applicable method to determine dioxins levels in fish and sea food samples. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| WOS研究方向 | Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45834]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Natl Res Ctr Environm Anal & Measurement, State Environm Protect Key Lab Dioxin Pollut Cont, Beijing 100029, Peoples R China 3.Comprehens Test Ctr Chinese Acad Inspect & Quaran, Beijing 100123, Peoples R China 4.Inner Mongolia Agr Univ, Coll Food Sci & Engn, Hohhot 010018, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Gangdou,Wang, Lingyun,Zhang, Songyan,et al. Simple and rapid determination of dioxin in fish and sea food using a highly sensitive reporter cell line, CBG 2.8D[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2021,100:353-359. |
| APA | Ding, Gangdou.,Wang, Lingyun.,Zhang, Songyan.,Li, Shuaizhang.,Xie, Qunhui.,...&Zhao, Bin.(2021).Simple and rapid determination of dioxin in fish and sea food using a highly sensitive reporter cell line, CBG 2.8D.JOURNAL OF ENVIRONMENTAL SCIENCES,100,353-359. |
| MLA | Ding, Gangdou,et al."Simple and rapid determination of dioxin in fish and sea food using a highly sensitive reporter cell line, CBG 2.8D".JOURNAL OF ENVIRONMENTAL SCIENCES 100(2021):353-359. |
入库方式： OAI收割
来源：生态环境研究中心
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