The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions
文献类型：期刊论文
| 作者 | Yan, Zhu-Ying; Chen, Jing; Shao, Jie; Jiao, Ze-Qing; Tang, Tian-Shu; Tang, Miao; Sheng, Zhi-Guo; Mao, Li; Huang, Rong; Huang, Chun-Hua |
| 刊名 | FREE RADICAL BIOLOGY AND MEDICINE
 |
| 出版日期 | 2021-08-01 |
| 卷号 | 171页码:69-79 |
| 关键词 | Ru(II) polypyridyl complex Photosensitizer Singlet oxygen Cellular uptake Ion-pairing Photodynamic therapy |
| ISSN号 | 0891-5849 |
| 英文摘要 | Developing the cell-impermeable Ru(II) polypyridyl cationic complexes as effective photosensitizers (PS) which have high cellular uptake and photo-toxicity, but low dark toxicity, is quite challenging. Here we found that the highly reactive singlet oxygen (1O2) can be generated by the irradiation of a typical Ru(II) polypyridyl complex Ru(II)tris(tetramethylphenanthroline) ([Ru(TMP)3]2+) under visible light irradiation by ESR with TEMPO (2,2,6,6-tetramethyl-4-piperidone-N-oxyl) as 1O2 probe. Effective cellular and nuclear delivery of cationic [Ru (TMP)3]2+ was achieved through our recently developed ion-pairing method, and 2,3,4,5-tetrachlorophenol (2,3,4,5-TeCP) was found to be the most effective among all chlorophenols tested. The accelerated cellular, especially nuclear uptake of [Ru(TMP)3]2+ results in the formation of 8-oxo-7,8-dihydro-2 '-deoxyguanosine (8oxodG) and DNA strand breaks, caspase 3/7 activation and cell apoptosis in HeLa cells upon light irradiation. More importantly, compared with other traditional photosensitizers, [Ru(TMP)3]2+ showed significant phototoxicity but low dark toxicity. Similar effects were observed when 2,3,4,5-TeCP was substituted by the currently clinically used anti-inflammatory drug flufenamic acid. This represents the first report that the cellimpermeable Ru(II) polypyridyl complex ion-paired with suitable lipophilic counter-anions functions as potent intracellular photosensitizer under visible light irradiation mainly via a 1O2-mediated mechanism. These findings should provide new perspectives for future investigations on other metal complexes with similar characteristics as promising photosensitizers for potential photodynamic therapy. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45855]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China 4.Chongqing Univ, Coll Bioengn, Minist Educ, Key Lab Biorheol Sci & Technol, Chongqing 400044, Peoples R China 5.Peking Univ Third Hosp, Dept Stomatol, Beijing 100191, Peoples R China 6.Res Ctr Ecoenvironm Sci, Joint Inst Environm Sci, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Zhu-Ying,Chen, Jing,Shao, Jie,et al. The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions[J]. FREE RADICAL BIOLOGY AND MEDICINE,2021,171:69-79. |
| APA | Yan, Zhu-Ying.,Chen, Jing.,Shao, Jie.,Jiao, Ze-Qing.,Tang, Tian-Shu.,...&Zhu, Ben-Zhan.(2021).The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions.FREE RADICAL BIOLOGY AND MEDICINE,171,69-79. |
| MLA | Yan, Zhu-Ying,et al."The cell-impermeable Ru(II) polypyridyl complex as a potent intracellular photosensitizer under visible light irradiation via ion-pairing with suitable lipophilic counter-anions".FREE RADICAL BIOLOGY AND MEDICINE 171(2021):69-79. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。