The critical role of superoxide anion radicals on delaying tetrachlorohydroquinone autooxidation by penicillamine
文献类型：期刊论文
| 作者 | Mao, Li; Huang, Chun-Hua; Shao, Bo; Qin, Li; Tang, Miao; Yan, Zhu-Ying; Liu, Zhi-Sheng; Shao, Jie; Sheng, Zhi-Guo; Zhu, Ben-Zhan |
| 刊名 | FREE RADICAL BIOLOGY AND MEDICINE
 |
| 出版日期 | 2021-02-01 |
| 卷号 | 163页码:369-378 |
| 关键词 | Superoxide anion radical O-2(center dot-) probe CT-02H Penicillamine Tetrachlorohydroquinone Autooxidation Ascorbate |
| ISSN号 | 0891-5849 |
| 英文摘要 | We have recently found that penicillamine, a classic copper-chelating thiol-drug for Wilson's disease, can delay tetrachlorohydroquinone (TCHQ) autooxidation via a previously unrecognized redox-activity. However, its underlying molecular mechanism remains not fully understood. In this study, we found, interestingly and unexpectedly, that superoxide dismutase (SOD) can significantly shorten the delay of TCHQ autooxidation by penicillamine, but not by ascorbate; SOD can also markedly increase the yields of the oxidized form of penicillamine. Similar effects were observed with a recently-developed specific and sensitive superoxide anion radical (O-2(center dot-)) probe CT-02H, which was also employed to successfully measure O-2(center dot-) generated from both TCHQ and TCHQ/penicillamine systems for the first time. More importantly, addition of extra O-2(center dot-) (KO2/18-crown-6) can further prolong the delaying effects by penicillamine and slow down penicillamine consumption. Taken together, an unexpected critical role of O-2(center dot-) in TCHQ/penicillamine interaction was proposed: O-2(center dot-) may regenerate penicillamine, thereby continuously reducing TCSQ(center dot-) to TCHQ and finally delaying TCHQ autooxidation; In contrast, if O-2(center dot-) were eliminated, which can not only markedly change the reaction equilibrium, accelerate the rate of interaction, and ultimately shorten the delay of TCHQ autooxidation by penicillamine, but can also accelerate penicillamine oxidation to form its corresponding disulfide solely via redox reaction without any minor nucleophilic reaction. These findings not only further support our previously-proposed redox mechanism for the protection against TCHQ-induced cytotoxicity by penicillamine, but also reveal a new mode of action for O-2(center dot-) in the inhibition of haloquinoids-induced toxicity by thiol antioxidants. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45856]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Res Ctr Ecoenvironm Sci & Hong Kong Baptist Univ, Joint Inst Environm Sci, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, Li,Huang, Chun-Hua,Shao, Bo,et al. The critical role of superoxide anion radicals on delaying tetrachlorohydroquinone autooxidation by penicillamine[J]. FREE RADICAL BIOLOGY AND MEDICINE,2021,163:369-378. |
| APA | Mao, Li.,Huang, Chun-Hua.,Shao, Bo.,Qin, Li.,Tang, Miao.,...&Zhu, Ben-Zhan.(2021).The critical role of superoxide anion radicals on delaying tetrachlorohydroquinone autooxidation by penicillamine.FREE RADICAL BIOLOGY AND MEDICINE,163,369-378. |
| MLA | Mao, Li,et al."The critical role of superoxide anion radicals on delaying tetrachlorohydroquinone autooxidation by penicillamine".FREE RADICAL BIOLOGY AND MEDICINE 163(2021):369-378. |
入库方式： OAI收割
来源：生态环境研究中心
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