A High-Throughput Platform for the Rapid Quantification of Phosphorylated Histone H2AX in Cell Lysates Based on Microplate Electrochemiluminescence Immunosensor Array
文献类型：期刊论文
| 作者 | Liu, Chang; Qie, Yu; Zhao, Lixia; Li, Minjie; Guo, Liang-Hong |
| 刊名 | ACS SENSORS
 |
| 出版日期 | 2021 |
| 页码 | - |
| 关键词 | Ninety-East Ridge (NER) temperature-salinity curve microbial vertical distribution water mass microbial diversity |
| ISSN号 | 2096-5508 |
| 英文摘要 | Although the microbial diversity of the Indian Ocean has been extensively investigated, little is known about the community composition of microbes in the Southern Indian Ocean. In the present study, we divided 60 water column samples on the Ninety-East Ridge (NER) into five water masses according to the temperature-salinity curves. We presented, for the first time, a full description of the microbial biodiversity on NER through high-throughput amplicon sequencing approach, including bacteria, archaea, and fungi. We found that bacteria exhibited higher richness and diversity than archaea and fungi across the water masses on NER. More importantly, each water mass on NER featured distinct prokaryotic microbial communities, as indicated by the results of non-metric multidimensional scaling. In contrast, fungi were eurybathic across the water masses. Redundancy analysis results demonstrated that environmental factors might play a pivotal role in the formation and stability of prokaryotic communities in each water mass, especially that of archaea. In addition, indicator species might be used as fingerprints to identify corresponding water masses on NER. These results provide new insights into the vertical distribution, structure, and diversity of microorganisms on NER from the perspective of water mass. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/46789]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310007, Peoples R China 4.China Jiliang Univ, Coll Qual & Safety Engn, Hangzhou 310018, Zhejiang, Peoples R China 5.China Jiliang Univ, Inst Environm & Hlth Sci, Hangzhou 310018, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chang,Qie, Yu,Zhao, Lixia,et al. A High-Throughput Platform for the Rapid Quantification of Phosphorylated Histone H2AX in Cell Lysates Based on Microplate Electrochemiluminescence Immunosensor Array[J]. ACS SENSORS,2021:-. |
| APA | Liu, Chang,Qie, Yu,Zhao, Lixia,Li, Minjie,&Guo, Liang-Hong.(2021).A High-Throughput Platform for the Rapid Quantification of Phosphorylated Histone H2AX in Cell Lysates Based on Microplate Electrochemiluminescence Immunosensor Array.ACS SENSORS,-. |
| MLA | Liu, Chang,et al."A High-Throughput Platform for the Rapid Quantification of Phosphorylated Histone H2AX in Cell Lysates Based on Microplate Electrochemiluminescence Immunosensor Array".ACS SENSORS (2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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