A Multiwell-Based Assay for Screening Thyroid Hormone Signaling Disruptors Using thibz Expression as a Sensitive Endpoint in Xenopus laevis
文献类型：期刊论文
| 作者 | Li, Jinbo ; Li, Yuanyuan; Zhu, Min; Song, Shilin; Qin, Zhanfen
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2022-02 |
| 卷号 | 27期号:3页码:798 |
| 关键词 | GENE-EXPRESSION BISPHENOL-A IN-VITRO POSTEMBRYONIC DEVELOPMENT VERTEBRATE DEVELOPMENT RECEPTOR DISRUPTORS METAMORPHOSIS ASSAY TRICLOSAN TETRABROMOBISPHENOL EXPOSURE |
| 英文摘要 | There is a need for rapidly screening thyroid hormone (TH) signaling disruptors in vivo considering the essential role of TH signaling in vertebrates. We aimed to establish a rapid in vivo screening assay using Xenopus laevis based on the T3-induced Xenopus metamorphosis assay we established previously, as well as the Xenopus Eleutheroembryonic Thyroid Assay (XETA). Stage 48 tadpoles were treated with a series of concentrations of T3 in 6-well plates for 24 h and the expression of six TH-response genes was analyzed for choosing a proper T3 concentration. Next, bisphenol A (BPA) and tetrabromobisphenol A (TBBPA), two known TH signaling disruptors, were tested for determining the most sensitive TH-response gene, followed by the detection of several suspected TH signaling disruptors. We determined 1 nM as the induction concentration of T3 and thibz expression as the sensitive endpoint for detecting TH signaling disruptors given its highest response to T3, BPA, and TBBPA. And we identified betamipron as a TH signaling agonist, and 2,2 ',4,4 '-tetrabromodiphenyl ether (BDE-47) as a TH signaling antagonist. Overall, we developed a multiwell-based assay for rapidly screening TH signaling disruptors using thibz expression as a sensitive endpoint in X. laevis. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47451]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Qin, Zhanfen |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jinbo,Li, Yuanyuan,Zhu, Min,et al. A Multiwell-Based Assay for Screening Thyroid Hormone Signaling Disruptors Using thibz Expression as a Sensitive Endpoint in Xenopus laevis[J]. MOLECULES,2022,27(3):798. |
| APA | Li, Jinbo,Li, Yuanyuan,Zhu, Min,Song, Shilin,&Qin, Zhanfen.(2022).A Multiwell-Based Assay for Screening Thyroid Hormone Signaling Disruptors Using thibz Expression as a Sensitive Endpoint in Xenopus laevis.MOLECULES,27(3),798. |
| MLA | Li, Jinbo,et al."A Multiwell-Based Assay for Screening Thyroid Hormone Signaling Disruptors Using thibz Expression as a Sensitive Endpoint in Xenopus laevis".MOLECULES 27.3(2022):798. |
入库方式： OAI收割
来源：生态环境研究中心
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