A Simple Structure-Switch Aptasensor Using Label-Free Aptamer for Fluorescence Detection of Aflatoxin B1
文献类型：期刊论文
| 作者 | Wang, Chao ; Yu, Hao; Zhao, Qiang
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2022-07 |
| 卷号 | 27期号:13页码:4257 |
| 关键词 | MYCOTOXINS SENSOR ASSAY PROBE B-1 |
| 英文摘要 | Aflatoxin B1 (AFB1) is one of the mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus, and it causes contamination in foods and great risk to human health. Simple sensitive detection of AFB1 is important and demanded for food safety and quality control. Aptamers can specifically bind to targets with high affinity, showing advantages in affinity assays and biosensors. We reported an aptamer structure-switch for fluorescent detection of aflatoxin B1 (AFB1), using a label-free aptamer, a fluorescein (FAM)-labeled complementary strand (FDNA), and a quencher (BHQ1)-labeled complementary strand (QDNA). When AFB1 is absent, these three strands assemble into a duplex DNA structure through DNA hybridization, making FAM close to BHQ1, and fluorescence quenching occurs. In the presence of AFB1, the aptamer binds with AFB1, instead of hybridizing with QDNA. Thus, FAM is apart from BHQ1, and fluorescence increases with the addition of AFB1. This assay allowed detection of AFB1 with a detection limit of 61 pM AFB1 and a dynamic concentration range of 61 pM to 4 mu M. This aptamer-based method enabled detection of AFB1 in complex sample matrix (e.g., beer and corn flour samples). |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47458]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Zhao, Qiang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chao,Yu, Hao,Zhao, Qiang. A Simple Structure-Switch Aptasensor Using Label-Free Aptamer for Fluorescence Detection of Aflatoxin B1[J]. MOLECULES,2022,27(13):4257. |
| APA | Wang, Chao,Yu, Hao,&Zhao, Qiang.(2022).A Simple Structure-Switch Aptasensor Using Label-Free Aptamer for Fluorescence Detection of Aflatoxin B1.MOLECULES,27(13),4257. |
| MLA | Wang, Chao,et al."A Simple Structure-Switch Aptasensor Using Label-Free Aptamer for Fluorescence Detection of Aflatoxin B1".MOLECULES 27.13(2022):4257. |
入库方式： OAI收割
来源：生态环境研究中心
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