A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency
文献类型：期刊论文
| 作者 | Huang, Xin ; Bashkenova, Nazym; Hong, Yantao; Lyu, Cong; Guallar, Diana; Hu, Zhe; Malik, Vikas; Li, Dan; Wang, Hailin; Shen, Xiaohua
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| 刊名 | CELL REPORTS
 |
| 出版日期 | 2022-06-07 |
| 卷号 | 39期号:10页码:110928 |
| ISSN号 | 2211-1247 |
| 关键词 | LONG NONCODING RNA TET PROTEINS ENHANCER ACTIVITY CHROMATIN BINDING METHYLATION PHASE PLURIPOTENCY PARASPECKLES RECRUITMENT |
| 英文摘要 | TET1 maintains hypomethylation at bivalent promoters through its catalytic activity in embryonic stem cells (ESCs). However, TET1 catalytic activity-independent function in regulating bivalent genes is not well understood. Using a proteomics approach, we map the TET1 interactome in ESCs and identify PSPC1 as a TET1 partner. Genome-wide location analysis reveals that PSPC1 functionally associates with TET1 and Polycomb repressive complex-2 (PRC2). We establish that PSPC1 and TET1 repress, and the lncRNA Neat1 activates, bivalent gene expression. In ESCs, Neat1 is preferentially bound to PSPC1 alongside its PRC2 association at bivalent promoters. During the ESC-to-epiblast-like stem cell (EpiLC) transition, PSPC1 and TET1 maintain PRC2 chromatin occupancy at bivalent gene promoters, while Neat1 facilitates the activation of certain bivalent genes by promoting PRC2 binding to their mRNAs. Our study demonstrates a TET1-PSPC1-Neat1 molecular axis that modulates PRC2-binding affinity to chromatin and bivalent gene transcripts in controlling stem cell bivalency. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47461]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Wang, Jianlong |
| 作者单位 | 1.Columbia Univ, Herbert Irving Comprehens Canc Ctr, Irving Med Ctr, Dept Med,Columbia Ctr Human Dev,Columbia Stem Cel, New York, NY 10032 USA 2.Tsinghua Univ, Tsinghua Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 4.Univ Santiago de Compostela, Ctr Res Mol Med & Chron Dis CiMUS, Santiago De Compostela 15782, Spain |
| 推荐引用方式 GB/T 7714 | Huang, Xin,Bashkenova, Nazym,Hong, Yantao,et al. A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency[J]. CELL REPORTS,2022,39(10):110928. |
| APA | Huang, Xin.,Bashkenova, Nazym.,Hong, Yantao.,Lyu, Cong.,Guallar, Diana.,...&Wang, Jianlong.(2022).A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.CELL REPORTS,39(10),110928. |
| MLA | Huang, Xin,et al."A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency".CELL REPORTS 39.10(2022):110928. |
入库方式： OAI收割
来源：生态环境研究中心
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