Aptamer fluorescence anisotropy assays for detection of aflatoxin B1 and adenosine triphosphate using antibody to amplify signal change
文献类型：期刊论文
| 作者 | Li, Yapiao; Yu, Hao ; Zhao, Qiang
|
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2022-03-01 |
| 卷号 | 12期号:12页码:7464-7468 |
| 关键词 | IN-VITRO SELECTION POLARIZATION IMMUNOASSAY AMPLIFICATION MOLECULES FOOD |
| 英文摘要 | Fluorescence polarization/anisotropy (FP/FA) is an attractive technology for determining small molecules in homogeneous solution based on rotation changes of a fluorescent reporter. Binding induced conformation change is a specific property of aptamers. This property has been integrated into aptamer based FA assays for small molecules. In this work, we reported aptamer FA assays for aflatoxin B1 (AFB1) and adenosine triphosphate (ATP) by using antibody conjugated complementary DNA at the 3 ' end and a fluorescein (FAM)-labeled aptamer at the 5 ' end. The hybridization of aptamer and cDNA induced a FAM label close to the large-sized antibody, which restricts the local rotation of FAM and gives high FA signal. With the addition of target, the aptamer probe binds with the target, and the aptamer-cDNA duplex is inhibited, causing FA signal decreases. This method achieved detection of 25 pM AFB1 and 1 mu M ATP, respectively. The assay is promising for application. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47470]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Zhao, Qiang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 3.UCAS, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yapiao,Yu, Hao,Zhao, Qiang. Aptamer fluorescence anisotropy assays for detection of aflatoxin B1 and adenosine triphosphate using antibody to amplify signal change[J]. RSC ADVANCES,2022,12(12):7464-7468. |
| APA | Li, Yapiao,Yu, Hao,&Zhao, Qiang.(2022).Aptamer fluorescence anisotropy assays for detection of aflatoxin B1 and adenosine triphosphate using antibody to amplify signal change.RSC ADVANCES,12(12),7464-7468. |
| MLA | Li, Yapiao,et al."Aptamer fluorescence anisotropy assays for detection of aflatoxin B1 and adenosine triphosphate using antibody to amplify signal change".RSC ADVANCES 12.12(2022):7464-7468. |
入库方式： OAI收割
来源：生态环境研究中心
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