Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds
文献类型：期刊论文
| 作者 | Xu, Hanqing ; Su, Jiahui; Ku, Tingting; Liu, Qian S.; Liang, Jiefeng; Ren, Zhihua; Zhou, Qunfang; Jiang, Guibin
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| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
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| 出版日期 | 2022-08-05 |
| 卷号 | 435期号:0页码:129024 |
| 关键词 | ACID RECEPTOR-ALPHA RETINOIC ACID GENE-EXPRESSION DEPENDENT TRANSCRIPTION GROWTH-INHIBITION IN-VITRO RXR CONTAMINANTS ANTAGONIST ACTIVATION |
| ISSN号 | 0304-3894 |
| 英文摘要 | The screening of compounds with endocrine disrupting effects has been attracting increasing attention due to the continuous release of emerging chemicals into the environment. Testing the (ant)agonistic activities of these chemicals on the retinoic acid receptor alpha (RAR alpha), a vital nuclear receptor, is necessary to explain their perturbation in the endocrine system in vivo. In the present study, MCF-7 breast carcinoma cells were transiently transfected with a RAR alpha expression vector (pEF1 alpha-RAR alpha-RFP) and a reporter vector containing a retinoic acid reaction element (pRARE-TA-Luc). Under optimized conditions, the performance of the newly constructed system was evaluated for its feasibility in screening the (ant)agonistic effects of emerging phenolic compounds on RAR alpha. The results showed that this transient transfection cell model responded well to stimulation with (ant) agonists of RAR alpha, and the EC50 and IC50 values were 0.87 nM and 2.67 mu M for AM580 and Ro41-5253, respectively. Its application in testing several emerging phenolic compounds revealed that triclosan (TCS) and tetrabromobisphenol A (TBBPA) exerted notable RAR alpha antagonistic activities. This newly developed bioassay based on MCF-7 is promising in identifying the agonistic or antagonistic activities of xenobiotics on RAR alpha and has good potential for studying RAR alpha signaling-involved toxicological effects of emerging chemicals of concern. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47491]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Zhou, Qunfang |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 2.Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China 3.Wenzhou Univ, Natl & Local Joint Engn Res Ctr Ecol Treatment Tec, Wenzhou 325035, Peoples R China 4.Shanxi Univ, Coll Environm & Resources, Res Ctr Environm & Hlth, Taiyuan 030006, Peoples R China 5.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hanqing,Su, Jiahui,Ku, Tingting,et al. Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds[J]. JOURNAL OF HAZARDOUS MATERIALS,2022,435(0):129024. |
| APA | Xu, Hanqing.,Su, Jiahui.,Ku, Tingting.,Liu, Qian S..,Liang, Jiefeng.,...&Jiang, Guibin.(2022).Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds.JOURNAL OF HAZARDOUS MATERIALS,435(0),129024. |
| MLA | Xu, Hanqing,et al."Constructing an MCF-7 breast cancer cell-based transient transfection assay for screening RAR? (Ant)agonistic activities of emerging phenolic compounds".JOURNAL OF HAZARDOUS MATERIALS 435.0(2022):129024. |
入库方式： OAI收割
来源：生态环境研究中心
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