Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution
文献类型：期刊论文
| 作者 | Kong, Yimeng; Cao, Lei ; Deikus, Gintaras; Fan, Yu; Mead, Edward A.; Lai, Weiyi; Zhang, Yizhou; Yong, Raymund; Sebra, Robert; Wang, Hailin
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| 刊名 | SCIENCE
 |
| 出版日期 | 2022-02-04 |
| 卷号 | 375期号:6580页码:515 |
| ISSN号 | 0036-8075 |
| 关键词 | SINGLE-MOLECULE RESTRICTION N-6-ADENINE GENES N-6-METHYLADENINE DATABASE |
| 英文摘要 | The discovery of N-6-methyldeoxyadenine (6mA) across eukaryotes led to a search for additional epigenetic mechanisms. However, some studies have highlighted confounding factors that challenge the prevalence of 6mA in eukaryotes. We developed a metagenomic method to quantitatively deconvolve 6mA events from a genomic DNA sample into species of interest, genomic regions, and sources of contamination. Applying this method, we observed high-resolution 6mA deposition in two protozoa. We found that commensal or soil bacteria explained the vast majority of 6mA in insect and plant samples. We found no evidence of high abundance of 6mA in Drosophila, Arabidopsis, or humans. Plasmids used for genetic manipulation, even those from Dam methyltransferase mutant Escherichia coli, could carry abundant 6mA, confounding the evaluation of candidate 6mA methyltransferases and demethylases. On the basis of this work, we advocate for a reassessment of 6mA in eukaryotes. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47494]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Fang, Gang |
| 作者单位 | 1.Icahn Sch Med Mt Sinai, Black Family Stem Cell Inst, New York, NY 10029 USA 2.Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY 10029 USA 3.Icahn Sch Med Mt Sinai, Icahn Inst Genom & Multiscale Biol, New York, NY 10029 USA 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 5.Icahn Sch Med Mt Sinai, Dept Neurosurg & Oncol Sci, New York, NY 10029 USA |
| 推荐引用方式 GB/T 7714 | Kong, Yimeng,Cao, Lei,Deikus, Gintaras,et al. Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution[J]. SCIENCE,2022,375(6580):515. |
| APA | Kong, Yimeng.,Cao, Lei.,Deikus, Gintaras.,Fan, Yu.,Mead, Edward A..,...&Fang, Gang.(2022).Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution.SCIENCE,375(6580),515. |
| MLA | Kong, Yimeng,et al."Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution".SCIENCE 375.6580(2022):515. |
入库方式： OAI收割
来源：生态环境研究中心
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