Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study
文献类型：期刊论文
| 作者 | Yang, Bingwei; Ye, Zhishuai; Wang, Yawen; Guo, Hongzhou; Lehmler, Hans-Joachim; Huang, Rongchong; Song, Erqun; Song, Yang
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| 刊名 | ENVIRONMENTAL HEALTH PERSPECTIVES
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| 出版日期 | 2022-03 |
| 卷号 | 130期号:3页码:37011 |
| ISSN号 | 0091-6765 |
| 关键词 | LONG NONCODING RNAS TGF-BETA CARDIOVASCULAR-DISEASE RISK-FACTORS CAVEOLIN-1 OBESITY SERUM PCBS INFLAMMATION ENDOTHELIUM |
| 英文摘要 | BACKGROUND: Miscellaneous cardiovascular risk factors have been defined, but the contribution of environmental pollutants exposure on cardiovascular disease (CVD) remains underappreciated. OBJECTIVE: We investigated the potential impact of typical environmental pollutant exposure on atherogenesis and its underlying mechanisms. METHODS: We used human umbilical vein endothelial cells (HUVECs) and apolipoprotein E knockout (ApoE(-/-)) mice to investigate how 2,3,5-trichloro-6-phenyl-[1,4]-benzoquinone (PCB29-pQ, a toxic polychlorinated biphenyl metabolite) affects atherogenesis and identified early biomarkers of CVD associated with PCB29-pQ exposures. Then, we used long noncoding RNAs (lncRNAs) HDAC7-A51-overexpressing ApoE(-/-) mice and apolipoprotein E/caveolin 1 double-knockout (ApoE(-/-)/CAV1(-/-))mice to address the role of these early biomarkers in PCB29-pQ-induced atherogenesis. Plasma samples from patients with coronary heart disease (CHD) were also used to confirm our findings. Results: Our data indicate that lncRNA HDAC7-A51 bound to MIR-7-5p via argonaute 2 in PCB29-pQ-challenged HUVECs. Our mRNA sequencing assay identified transforming growth factor-beta 2 (TGF-beta 2) as a possible target gene of MIR-7-5p; HDAC7-AS1 sponged MIR-7-5p and inhibited the binding of TGF-beta 2 to MIR-7-5p. The effect of PCB29-pQ-induced endothelial injury, vascular inflammation, development of plaques. and atherogenesis in ApoE(-)(/-), mice was greater with MIR-7-5p-mediated TGF-beta 2 inhibition, whereas HDAC7-AS1-overexpressing ApoE(-/-) mice and ApoE(-/-)/CAV1(-/-) mice showed the opposite effect. Consistently, plasma levels of HDAC7-A51 and MIR-7-5p were found to be significantly associated individuals diagnosed with CHD. DISCUSSIONS: These findings demonstrated that a mechanism-based, integrated-omics approach enabled the identification of potentially clinically relevant diagnostic indicators and therapeutic targets of CHD mediated by environmental contaminants using in vitro and in vivo models of HUVECs and ApoE(-/-) and ApoE(-/-)/CAV1(-/-) mice. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47531]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 通讯作者 | Song, Yang |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China 2.Capital Med Univ, Beijing Friendship Hosp, Dept Cardiol, Beijing, Peoples R China 3.Univ Iowa, Dept Occupat & Environm Hlth, Iowa City, IA USA 4.Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Minist Educ, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Bingwei,Ye, Zhishuai,Wang, Yawen,et al. Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2022,130(3):37011. |
| APA | Yang, Bingwei.,Ye, Zhishuai.,Wang, Yawen.,Guo, Hongzhou.,Lehmler, Hans-Joachim.,...&Song, Yang.(2022).Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study.ENVIRONMENTAL HEALTH PERSPECTIVES,130(3),37011. |
| MLA | Yang, Bingwei,et al."Evaluation of Early Biomarkers of Atherosclerosis Associated with Polychlorinated Biphenyl Exposure: An in Vitro and in Vivo Study".ENVIRONMENTAL HEALTH PERSPECTIVES 130.3(2022):37011. |
入库方式： OAI收割
来源：生态环境研究中心
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