Sensitive determination of metalloprotein in salt-rich matrices by size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry
文献类型：期刊论文
| 作者 | Tang, Yinyin ; Liu, Lihong; Nong, Qiying; Guo, Hua; Zhou, Qinfei; Wang, Dingyi; Yin, Yongguang; Shi, Jianbo; He, Bin; Hu, Ligang
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
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| 出版日期 | 2022-08-16 |
| 卷号 | 1677期号:0页码:463303 |
| ISSN号 | 0021-9673 |
| 关键词 | FLOW GEL-ELECTROPHORESIS ORYZA-SATIVA L. HPLC-ICP-MS CAPILLARY-ELECTROPHORESIS CONTAINING PROTEINS BINDING-PROPERTIES E. COLI MERCURY IDENTIFICATION SPECIATION |
| 英文摘要 | Metalloproteins play crucial and distinct roles in a variety of biological processes that rely heavily on the metal ions and various proteins. However, there is still a lack of method for rapid analysis of metalloproteins in complex samples, especially in salt-rich matrices. In this study, a sensitive method for separation and determination of metalloproteins in salt-rich matrices was developed based on the size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry (SEC-ICP-MS), combining with the high matrix introduction (HMI) mode, which is quite essential for biological system. The separation conditions of the SEC-ICP-MS system were optimized by using four iodine labeled proteins with different molecular weights, including bovine serum albumin (BSA, 66.0 kDa), ovalbumin (OVA, 44.0 kDa), carbonic anhydrase (CA, 29.0 kDa) and ribonuclease A (RA, 13.7 kDa). After optimization, four iodine labeled proteins and iodine ions were successfully separated within 30 min by using 10 mmol/L HEPES and 40 mmol/L Na2SO4 (pH=7.0) as mobile phase and a linear relationship between log molecular weight and retention time was established. The relative standard deviations (RSDs, n = 5) of the retention time and peak areas for the four iodine labeled proteins were in the range of 0.2-0.9% and 3.3-7.7%, respectively, suggesting good precision and repeatability. Then the proposed method was successfully applied to the rapid separation and detection of lead-binding proteins in real biological tissue samples. (C) 2022 Published by Elsevier B.V. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47616]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 2.Jianghan Univ, Sch Environm & Hlth, Hubei Key Lab Environm & Hlth Effects Persistent T, Wuhan 4 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Environm, Hangzhou 310024, Peoples R China 4.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 5.Guangdong Prov Hosp Occupat Dis Prevent & Treatmen, Guangzhou 510300, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Yinyin,Liu, Lihong,Nong, Qiying,et al. Sensitive determination of metalloprotein in salt-rich matrices by size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2022,1677(0):463303. |
| APA | Tang, Yinyin.,Liu, Lihong.,Nong, Qiying.,Guo, Hua.,Zhou, Qinfei.,...&Jiang, Guibin.(2022).Sensitive determination of metalloprotein in salt-rich matrices by size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1677(0),463303. |
| MLA | Tang, Yinyin,et al."Sensitive determination of metalloprotein in salt-rich matrices by size exclusion chromatography coupled with inductively coupled plasma-mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1677.0(2022):463303. |
入库方式： OAI收割
来源：生态环境研究中心
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