Structural Insights into the Mechanism of High-Affinity Binding of Ochratoxin A by a DNA Aptamer
文献类型：期刊论文
| 作者 | Xu, Guohua; Zhao, Jiajing; Yu, Hao ; Wang, Chen; Huang, Yangyu; Zhao, Qiang; Zhou, Xin; Li, Conggang; Liu, Maili
|
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2022-05-04 |
| 卷号 | 144期号:17页码:7731-7740 |
| ISSN号 | 0002-7863 |
| 关键词 | XPLOR-NIH RNA FLUORESCENCE ASSIGNMENT |
| 英文摘要 | A 36-mer guanine (G)-rich DNA aptamer (OBA36) is able to distinguish one atomic difference between ochratoxin analogues A(OTA) and B (OTB), showing prominent recognition specificity andaffinity among hundreds of aptamers for small molecules. Why OBA36has >100-fold higher binding affinity to OTA than OTB remains a long-standing question due to the lack of high-resolution structure. Here wereport the solution NMR structure of the aptamer-OTA complex. It wasfound that OTA binding induces the aptamer to fold into a well-definedunique duplex-quadruplex structural scaffold stabilized by Mg2+and Na+ions. OTA does not directly interact with the G-quadruplex, butspecifically binds at the junction between the double helix and G-quadruplex through pi-pi stacking, halogen bonding (X-bond), andhydrophobic interaction. OTB has the same binding site as OTA butlacks the X-bond. The strong X-bond formed between the chlorine atom of OTA and the aromatic ring of C5 is the key todiscriminating the strong binding toward OTA. The present research contributes to a deeper insight of aptamer molecularrecognition, reveals structural basis of the high-affinity binding of aptamers, and provides a foundation for further aptamer engineering and applications |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/47621]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Xian Modern Chem Res Inst, Xian 710065, Peoples R China 4.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Innovat Acad Precis Measurement Sci & Technol,Wuh, Natl Ctr Magnet Resonance Wuhan,Wuhan Natl Lab Op, Wuhan 430071, Peoples R China 5.UCAS, Hangzhou Inst Adv Study, Hangzhou 310000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Guohua,Zhao, Jiajing,Yu, Hao,et al. Structural Insights into the Mechanism of High-Affinity Binding of Ochratoxin A by a DNA Aptamer[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2022,144(17):7731-7740. |
| APA | Xu, Guohua.,Zhao, Jiajing.,Yu, Hao.,Wang, Chen.,Huang, Yangyu.,...&Liu, Maili.(2022).Structural Insights into the Mechanism of High-Affinity Binding of Ochratoxin A by a DNA Aptamer.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,144(17),7731-7740. |
| MLA | Xu, Guohua,et al."Structural Insights into the Mechanism of High-Affinity Binding of Ochratoxin A by a DNA Aptamer".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 144.17(2022):7731-7740. |
入库方式： OAI收割
来源：生态环境研究中心
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