An Improved Multiple Competitive Immuno-SERS Sensing Platform and Its Application in Rapid Field Chemical Toxin Screening
文献类型：期刊论文
| 作者 | Sun, Jiefang; Wang, Zixuan ; Yang, Ling; He, Yi; Liu, Rui; Ran, Wei; Wang, Zhanhui; Shao, Bing
|
| 刊名 | TOXICS
 |
| 出版日期 | 2022 |
| 卷号 | 10期号:10页码:605-1-11 |
| 关键词 | multiple immunoassay SERS assay rodenticides non-fouling interface |
| 英文摘要 | Improving the signal-to-noise ratio (SNR) by amplifying the outputting signal or reducing nonspecific binding (NSB) are the key techniques in multiple immunoassay. Aiming at these issues, this paper presents an improved multiple indirect competitive immune surface-enhanced Raman scattering (ci-SERS) assay for the rapid screening of highly toxic rodenticides in food and biological samples, which ensured remarkable accuracy, ultra-sensitivity and reproducibility. The non-fouling polymer brush grafted magnetic beads (the MB@P-CyM) were prepared as multiple competitive recognition substrates after conjugating triplex haptens (the MB@P-CyM-hap). It was demonstrated that the particular 3D hair-like structures of P-CyM not only facilitate conjugate high-density hapten but reduce the steric hindrance from SERS probes recognition, thus enhancing SNB. On the other hand, Au nanoflowers (AuNFs) of high SERS activity were synthesized using a simple one-pot hydrazine reduction. For simultaneously detecting three highly toxic rodenticides, i.e., diphacinone (DPN), bromadiolone (BRD) and tetramine (TET), the obtained AuNFs were fabricated as a SERS-encoded nanoprobe cocktail after successively labeling mono-antibodies/Raman probes. By integrating the MB@P-CyM-hap with the SERS-encoded cocktail, a highly sensitive multiple SERS assay was achieved in less than 2 h with a limit of detection of 0.62 ng mL(-1) for BRD, 0.42 ng mL(-1) for TET and 1.37 ng mL(-1) for DPN, respectively. The recoveries of these rodenticides in spiked food and biological samples were determined and ranged from 72 to 123%. Above all, the proposed modifications show remarkable improvements for high efficient multiple chemical toxin immunoassay. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/48476]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.China Agricultural University 2.Southwest University of Science & Technology - China 3.Research Center for Eco-Environmental Sciences (RCEES) 4.Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Sun, Jiefang,Wang, Zixuan,Yang, Ling,et al. An Improved Multiple Competitive Immuno-SERS Sensing Platform and Its Application in Rapid Field Chemical Toxin Screening[J]. TOXICS,2022,10(10):605-1-11. |
| APA | Sun, Jiefang.,Wang, Zixuan.,Yang, Ling.,He, Yi.,Liu, Rui.,...&Shao, Bing.(2022).An Improved Multiple Competitive Immuno-SERS Sensing Platform and Its Application in Rapid Field Chemical Toxin Screening.TOXICS,10(10),605-1-11. |
| MLA | Sun, Jiefang,et al."An Improved Multiple Competitive Immuno-SERS Sensing Platform and Its Application in Rapid Field Chemical Toxin Screening".TOXICS 10.10(2022):605-1-11. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。