Aptamer Molecular Beacon Sensor for Rapid and Sensitive Detection of Ochratoxin A
文献类型：期刊论文
| 作者 | Yu, Hao ; Zhao, Qiang
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2022 |
| 卷号 | 27期号:23页码:8267-1-9 |
| 关键词 | ochratoxin a aptamer fluorescence molecular beacon sensor |
| 英文摘要 | Ochratoxin A (OTA) is a carcinogenic fungal secondary metabolite which causes wide contamination in a variety of food stuffs and environments and has a high risk to human health. Developing a rapid and sensitive method for OTA detection is highly demanded in food safety, environment monitoring, and quality control. Here, we report a simple molecular aptamer beacon (MAB) sensor for rapid OTA detection. The anti-OTA aptamer has a fluorescein (FAM) labeled at the 5 ' end and a black hole quencher (BHQ1) labeled at the 3 ' end. The specific binding of OTA induced a conformational transition of the aptamer from a random coil to a duplex-quadruplex structure, which brought FAM and BHQ1 into spatial proximity causing fluorescence quenching. Under the optimized conditions, this aptamer sensor enabled OTA detection in a wide dynamic concentration range from 3.9 nM to 500 nM, and the detection limit was about 3.9 nM OTA. This method was selective for OTA detection and allowed to detect OTA spiked in diluted liquor and corn flour extraction samples, showing the capability for OTA analysis in practical applications. |
| 源URL | [https://ir.rcees.ac.cn/handle/311016/48477]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 作者单位 | 1.Chinese Academy of Sciences 2.Research Center for Eco-Environmental Sciences (RCEES) 3.University of Chinese Academy of Sciences, CAS |
| 推荐引用方式 GB/T 7714 | Yu, Hao,Zhao, Qiang. Aptamer Molecular Beacon Sensor for Rapid and Sensitive Detection of Ochratoxin A[J]. MOLECULES,2022,27(23):8267-1-9. |
| APA | Yu, Hao,&Zhao, Qiang.(2022).Aptamer Molecular Beacon Sensor for Rapid and Sensitive Detection of Ochratoxin A.MOLECULES,27(23),8267-1-9. |
| MLA | Yu, Hao,et al."Aptamer Molecular Beacon Sensor for Rapid and Sensitive Detection of Ochratoxin A".MOLECULES 27.23(2022):8267-1-9. |
入库方式： OAI收割
来源：生态环境研究中心
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