Genotoxicity detection of oil-containing drill cuttings by Comet assay based on a demersal marine fish Mugilogobius chulae
文献类型：期刊论文
| 作者 | Cui, Zhisong; Luan, Xiao; Li, Shujun; Zhao, Xilong; Lin, Zhongting; Li, Jianjun; Gao, Wei ; Zheng, Li; Ma, Zhengzhao; Xie, Jianghao
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| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2021-01-15 |
| 卷号 | 208页码:- |
| 关键词 | Drill cuttings Genotoxicity Comet assay Single cell gel electrophoresis (SCGE) Mugilogobius chulae |
| ISSN号 | 0147-6513 |
| 英文摘要 | An enormous amount of oil-containing drill cuttings have been produced by the marine oil and gas industry. The environmental impacts of discharged drilling waste have been extensively studied. However, there is still an urgent need to develop alternative methods to identify the genotoxicity of untreated and treated drill waste in a timely manner before it is discharged. In this study, we developed a relatively rapid, sensitive, and accurate genotoxicity-detection method using Comet assay and the marine benthic goby Mugilogobius chulae. This goby is sensitive to a standard toxicant mitomycin C (MMC). The optimal exposure period for genotoxicity detection using M. chulae was determined. Three genotoxic indices (tail length (TL), tail DNA content (TD), and tail moment (TM)) were used to assess the effectiveness of high-temperature treatment of oil-contaminated waste. Untreated oil-containing drill cuttings exhibited the highest genotoxicity to goby cells. Genotoxicity was dramatically reduced after thermal treatment of drill cuttings at 350 degrees C and 500 degrees C. TD and TM exhibited significant correlation with the concentration of total petroleum hydrocarbons (TPHs)/total polycyclic aromatic hydrocarbons (PAHs) according to Pearson and Mantel correlation analyses (P values were < 0.05). Using redundancy analysis (RDA) and variation partition analysis (VPA), the genotoxic effects of the drill cuttings were ascribed to total alkanes and specific groups of PAHs. In conclusion, this newly established biological model has the potential to be widely used to detect the genetic damage of untreated or treated oil-containing drill cuttings discharged into the marine environment. |
| WOS研究方向 | Environmental Sciences ; Toxicology |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45947]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 作者单位 | 1.Minist Nat Resources China, Inst Oceanog 1, Marine Bioresources & Environm Res Ctr, Qingdao 266061, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China 4.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China 5.Guangdong Lab Anim Monitoring Inst, Key Lab Guangdong Lab Anim, Guangzhou 510663, Peoples R China 6.China Offshore Environm Serv Co Ltd, China Natl Offshore Oil Corp, CNOOC Energy Technol |
| 推荐引用方式 GB/T 7714 | Cui, Zhisong,Luan, Xiao,Li, Shujun,et al. Genotoxicity detection of oil-containing drill cuttings by Comet assay based on a demersal marine fish Mugilogobius chulae[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,208:-. |
| APA | Cui, Zhisong.,Luan, Xiao.,Li, Shujun.,Zhao, Xilong.,Lin, Zhongting.,...&Xie, Jianghao.(2021).Genotoxicity detection of oil-containing drill cuttings by Comet assay based on a demersal marine fish Mugilogobius chulae.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,208,-. |
| MLA | Cui, Zhisong,et al."Genotoxicity detection of oil-containing drill cuttings by Comet assay based on a demersal marine fish Mugilogobius chulae".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 208(2021):-. |
入库方式： OAI收割
来源：生态环境研究中心
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