Potential dissemination mechanism of the tetC gene in Aeromonas media from the aerobic biofilm reactor under oxytetracycline stresses
文献类型：期刊论文
| 作者 | Shi, Yanhong; Zhang, Yu ; Wu, Xiangyang; Zhang, Hong; Yang, Min; Tian, Zhe
|
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2021-07 |
| 卷号 | 105页码:90-99 |
| 关键词 | Aeromonas media tetC IS26 Circular intermediate Inverse PCR |
| ISSN号 | 1001-0742 |
| 英文摘要 | The tetC gene has been found to be one of the most widely distributed tetracycline resistance (tet) genes in various environmental niches, but the detailed dissemination mechanisms are still largely unknown. In the present study, 11 tetC-containing Aeromonas media strains were isolated from an aerobic biofilm reactor under oxytetracycline stresses, and the genome of one strain was sequenced using the PacBio RSII sequencing approach to reveal the genetic environment of tetC. The tetC gene was carried by an IS26 composite transposon, named Tn6434. The tetC-carrying Tn6434 structure was detected in all of the A. media strains either in a novel plasmid pAeme2 (n=9) or other DNA molecules (n=2) by PCR screening. The NCBI database searching result shows that this structure was also present in the plasmids or chromosomes of other 13 genera, indicating the transferability of Tn6434. Inverse PCR and sequencing confirmed that Tn6434 can form a circular intermediate and is able to incorporate into a preexisting IS26 element, suggesting that Tn6434 might be responsible for the dissemination of tetC between different DNA molecules. This study will be helpful in uncovering the spread mechanism of tet genes in water environments. (C) 2021 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| WOS研究方向 | Environmental Sciences |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45977]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 作者单位 | 1.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China 2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yanhong,Zhang, Yu,Wu, Xiangyang,et al. Potential dissemination mechanism of the tetC gene in Aeromonas media from the aerobic biofilm reactor under oxytetracycline stresses[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2021,105:90-99. |
| APA | Shi, Yanhong,Zhang, Yu,Wu, Xiangyang,Zhang, Hong,Yang, Min,&Tian, Zhe.(2021).Potential dissemination mechanism of the tetC gene in Aeromonas media from the aerobic biofilm reactor under oxytetracycline stresses.JOURNAL OF ENVIRONMENTAL SCIENCES,105,90-99. |
| MLA | Shi, Yanhong,et al."Potential dissemination mechanism of the tetC gene in Aeromonas media from the aerobic biofilm reactor under oxytetracycline stresses".JOURNAL OF ENVIRONMENTAL SCIENCES 105(2021):90-99. |
入库方式： OAI收割
来源：生态环境研究中心
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